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Bobust Binding Proteins of Predetermined Specificity

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1 R43 AI38735-01,
Agency Tracking Number: 29150
Amount: $100,000.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 1995
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
99-193 Aiea Heights Drive
Aiea, HI 96701
United States
DUNS: N/A
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Iain Peters
 () -
Business Contact
Phone: () -
Research Institution
N/A
Abstract

We will develop a specific binding protein that has antibody-like diversity but is suitable for higexpression and use in extreme environments. Production of recombinant antibodies in E. coli providessource of active protein for antibody-based technologies, and the small size of the recombinant antisignificant advantages in some applications. However, there are disadvantages attributable to the macomplicated dimeric peptide structure of recombinant antibodies. The bacterial folding and secretionyield high levels of functional expression, especially in the case of Fab. Also, the weakness of assvariable domains often leads to stability problems, especially in case of single- chain Fv. This spean antigen binding site similar to that of antibodies in aspects important for binding and diversitybacterial protein of high thermostability. The design is monomeric, and thus should circumvent foldistability problems associated with recombinant antibody fragments. It should be possible to recoverdesired specificity┬┐s from a diverse library of these molecules by phage display technology.

* Information listed above is at the time of submission. *

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