Bobust Binding Proteins of Predetermined Specificity

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1 R43 AI38735-01,
Agency Tracking Number: 29150
Amount: $100,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1995
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
99-193 Aiea Heights Drive, Aiea, HI, 96701
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Iain Peters
 () -
Business Contact
Phone: () -
Research Institution
We will develop a specific binding protein that has antibody-like diversity but is suitable for higexpression and use in extreme environments. Production of recombinant antibodies in E. coli providessource of active protein for antibody-based technologies, and the small size of the recombinant antisignificant advantages in some applications. However, there are disadvantages attributable to the macomplicated dimeric peptide structure of recombinant antibodies. The bacterial folding and secretionyield high levels of functional expression, especially in the case of Fab. Also, the weakness of assvariable domains often leads to stability problems, especially in case of single- chain Fv. This spean antigen binding site similar to that of antibodies in aspects important for binding and diversitybacterial protein of high thermostability. The design is monomeric, and thus should circumvent foldistability problems associated with recombinant antibody fragments. It should be possible to recoverdesired specificity¿s from a diverse library of these molecules by phage display technology.

* Information listed above is at the time of submission. *

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government