MONOCLONAL AND POLYCLONAL MAGAKARYOCYTE CSF ANTIBODIES

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$500,000.00
Award Year:
1993
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
19093
Agency Tracking Number:
19093
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Hipple Cancer Research Center (Currently Hipple Cancer Research)
4100 South Kettering Boulevard, Dayton, OH, 45439
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Martin J Murphy Jr
(513) 299-7120
Business Contact:
() -
Research Institution:
n/a
Abstract
THE PRODUCTION OF MEGAKARYOCYTES FROM PROGENITOR CELLS IN THE MARROW CAN BE STIMULATED BY A LINEAGE-SPECIFIC FACTOR-MEGAKARYOCYTE COLONY-STIMULATING FACTOR (MEG-CSF). THE LINEAGE-SPECIFIC STIMULATION OF MEGAKARYOCYTES BY MEG-CSF AND SUBSEQUENT INCREASE IN PLATELET NUMBER HAS GREAT CLINICAL SIGNIFICANCE IN TREATING THROMBOCYTOPENIA RESULTING FROM ANTI-CANCER OR ANTI-HIV THERAPIES. THE PRODUCTION OF POLYCLONAL AND MONOCLONAL ANTIBODIES TO MEG-CSF WOULD FACILITATE THE PURIFICATION OF MEG-CSF BY AFFINITY CHROMOTOGRAPHY, AND THE IDENTIFICATION OF RECOMBINANT CLONES EXPRESSING MEG-CSF CDNA. IN ADDITION, SUCH ANTIBODIES WILL ENABLE THE DEVELOPMENT OF ASSAYS, SUCH AS AN ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA), TO DETERMINE SERUM OR URINE MEG-CSF LEVELS IN THOSE PATIENTS UNDERGOING CHEMOTHERAPY OR WHO HAVE THROMBOCYTOPENIA FROM OTHER CAUSES. RABBITS WILL BE IMMUNIZED TO OBTAIN POLYCLONAL ANTIBODIES AND BOTH IN VITRO AND IN VIVO IMMUNIZATION OF MICE WILL BE PERFORMED FOR MONOCLONAL ANTIBODY PRODUCTION.

* information listed above is at the time of submission.

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