CONSTRUCTION OF CHICKEN X MOUSE HYBRIDOMAS

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 18921
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1992
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Houston Biotechnology
3608 Research Forest Drive, The Woodlands, TX, 77381
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Peter J Kelleher
 (713) 363-0999
Business Contact
Phone: () -
Research Institution
N/A
Abstract
WE WILL CONSTRUCT CHICKEN KH MOUSE HYBRIDOMA CELL LINES SECRETING CHICKEN MABS TO SELECTED ANTIGENS. A PRIMARY ADVANTAGE IN THE CONSTRUCTION OF THESE HYBRIDOMAS IS THE GENETIC DIFFERENCES BETWEEN CHICKENS AND MAMMALS WHICH INCREASES THE CHANCE OF PRODUCING ANTIBODIES AGAINST HIGHLY CONSERVED REGIONS OF MAMMALIAN PROTEINS SUCH AS PEPTIDE HORMONES AND GROWTH FACTORS. BECAUSE OF THIS GENETIC DIFFERENCE, CHICKEN MABS COULD POTENTIALLY BE PREPARED TO EPITOPES THAT ARE EITHER WEAKLY OR NON-IMMUNOGENIC IN MICE OR RATS. INTERSPECIES HYBRIDOMAS HAVE BEEN PREPARED BY FUSING IMMUNE LYMPHOCYTES FROM A NUMBER OF MAMMALIAN SPECIES WITH MOUSE MYELOMA CELLS. THESE HYBRIDOMAS ARE PRIMARILY USEFUL FOR STUDIES UNIQUE TO THAT SPECIES. HOWEVER, CHICKEN MABS TECHNOLOGY COULD POTENTIALLY BECOME AS IMPORTANT AS CURRENT MOUSE AND RATMAB TECHNOLOGY. THE MAJOR OBJECTIVE OF PHASE I IS TO EXAMINE THE FEASIBILITY AND OPTIMAL CONDITIONS FOR THE PREPARATION OF CHICKEN KH MOUSE HYBRIDOMAS SECRETING CHICKEN MABS TO A SELECTED MODEL ANTIGEN. THE HYBRIDOMAS WILL BE MONITORED BY STABILITY, GROWTH AND IMMUNOGLOBULIN PRODUCTION. SINCE IT HAS BEEN DEMONSTRATED THAT INTERSPECIES HYBRIDOMAS PREPARED WITH MOUSE MYELOMA CELL LINES ARE GENERALLY UNSTABLE, A SECONDARY OBJECTIVE WILL INCLUDE THE EXAMINATION OF SELECTIVE CHICKEN KH MOUSE HETEROMYELOMAS AS FUSION PARTNERS THAT WILL SUPPORT STABLE HYBRIDOMAS WHEN FUSED WITH IMMUNE CHICKEN LYMPHOCYTES.

* information listed above is at the time of submission.

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