OPTIMIZING MULTIPARAMETER FLOW CYTOMETRY OF TUMOR CELLS

Award Information
Agency:
Department of Health and Human Services
Amount:
$44,000.00
Program:
SBIR
Contract:
N/A
Solitcitation Year:
N/A
Solicitation Number:
N/A
Branch:
N/A
Award Year:
1987
Phase:
Phase I
Agency Tracking Number:
7139
Solicitation Topic Code:
N/A
Small Business Information
Howard M Shapiro Md Pc
283 Highland Avenue, West Newton, MA, 02165
Hubzone Owned:
N
Woman Owned:
N
Socially and Economically Disadvantaged:
N
Duns:
N/A
Principal Investigator
 HOWARD M SHAPIRO MD
 (617) 965-6044
Business Contact
Phone: () -
Research Institution
N/A
Abstract
FLOW CYTOMETRY OF SUCH PARAMETERS AS DNA, RNA, SURFACE AND/OR INTERNAL ANTIGEN, AND TOTAL PROTEIN CONTENT OF TUMOR CELLS OR NUCLEI IS WIDELY USED IN CANCER RESEARCH AND IS FINDING INCREASING CLINICAL APPLICATION. USEFUL INFORMATIONABOUT THE BIOLOGY OF NORMAL AND ABNORMAL CELLULAR DEVELOPMENT, THE PERTURBATION OF CELL FUNCTION BY THERAPEUTIC AGENTS, AND THE BIOLOGIC BEHAVIOR OF TUMORS IN PATIENTS, OBTAINED FROM ONE-AND TWO-PARAMETER FLOW CYTOMETRY, HAS LED INVESTIGATORS TO ATTEMPT MORE SOPHISTICATED STUDIES IN WHICH DNA, RNA, ONE OR MORE CELLULAR ANTIGENS, AND LIGHT SCATTERING OR EXTINCTION ARE MEASURED SIMULTANEOUSLY. THE MULTIPLE-BEAM APPARATUS AND MANY OF THE STAINING PROCEDURES USABLE FOR SUCH ANALYSES HAVE BEEN DEVELOPED; HOWEVER, THE TECHNIQUES HAVE REMAINED INACCESSIBLE TO MANY INVESTIGATORS BECAUSE OF THE DIFFICULTY AND COST OF IMPLEMENTING THEM ON CONVENTIONAL COMMERCIAL FLOW CYTOMETERS. THE PRESENT STUDY WILL EXAMINE THE PERFORMANCE OF TWO NEWER FLOW CYTOMETERS USING LOW-POWER LASER LIGHT SOURCES IN MULTIPARAMETER ANALYSIS OF DNA, RNA, AND IMMUNOFLUORESCENCE AND/OR PROTEIN CONTENT IN CELLS DERIVED FROM HUMAN TUMORS. THESE INSTRUMENTS, POTENTIALLY MUCH MORE AFFORDABLE AND EASIER TO USE THAN EXISTING SYSTEMS, COULD MAKE IT POSSIBLE FOR MANY MORE LABORATORIES TO PURSUE CLINICAL AND EXPERIMENTAL APPLICATIONS OF MULTIPARAMETER FLOW CYTOMETRY.

* information listed above is at the time of submission.

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