OPTIMIZATION AND EVALUATION OF AN ACROSOME ASSAY

Award Information
Agency:
Department of Health and Human Services
Branch:
N/A
Amount:
$50,000.00
Award Year:
1989
Program:
SBIR
Phase:
Phase I
Contract:
N/A
Agency Tracking Number:
11193
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Humagen Inc
1500 Avon St Ext, Charlottesville, VA, 22901
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
N/A
Principal Investigator
 () -
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE ABILITY TO MEASURE AN ACROSOME REACTION IN HUMAN SPERM MAY LEAD TO NEW DIAGNOSES AND THERAPY FOR MALE INFERTILITY. A NUMBER OF TECHNIQUES HAVE BEEN DESCRIBED, BUT NONE ARE ROUTINELY USED IN A CLINICAL SEMEN ANALYSIS. THE OBJECTIVE OF THIS RESEARCH IS TO OPTIMIZE THE CONDITIONSFOR AN INDIRECT IMMUNOFLUORESCENCE ASSAY USING EXISTING MONOCLONAL ANTIBODIES, T6 AND MHS-10, TO QUANTITATE ACROSOME-REACTED SPERM. ASSAYS WILL BE DEVELOPED FOR DETERMINING THE ACROSOME STATUS OF A FRESH EJACULATE AS WELLAS FOR MEASURING THE ACROSOME-INDUCIBLE POPULATION IN A SEMEN SPECIMEN. THE ASSAY WILL BE OPTIMIZED FOR MINIMAL DAY-TO-DAY AND DONOR-TO-DONOR VARIABILITY. THE INDIRECT IMMUNOFLUORESCENCE ASSAY WILL ALSO BE EVALUATED USING AN ACROSOME INDUCTION TECHNIQUE THAT HAS BEEN EXTENSIVELY CORRELATED WITH IN VITRO FERTILIZATION AND PREGNANCY RATES. IF AN ACROSOME-REACTED POPULATION CAN BE CONSISTENTLY DETECTED, THE ASSAY WILL BE MOVED INTO CLINICAL TRIALS AT BAYLOR COLLEGE OF MEDICINE TO EVALUATE SPERM FROM INFERTILE PATIENTS.

* information listed above is at the time of submission.

Agency Micro-sites

US Flag An Official Website of the United States Government