Phase plate holder for transmission electron microscopy

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$100,000.00
Award Year:
2009
Program:
SBIR
Phase:
Phase I
Contract:
1R43GM085841-01A1
Agency Tracking Number:
GM085841
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
HUMMINGBIRD SCIENTIFIC, LLC
HUMMINGBIRD PRECISION MACHINE CO, 8300 28TH COURT NE STE 200, LACEY, WA, 98516
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
171838019
Principal Investigator:
NORMAN SALMON
(360) 252-2737
NORMAN@HUMMINGBIRDSCIENTIFIC.COM
Business Contact:
NORMAN SALMON
() -
norman@hummingbirdscientific.com
Research Institution:
n/a
Abstract
DESCRIPTION (provided by applicant): The objective of our application is to develop hardware and procedures that will allow researchers to practically utilize phase plates in the back focal plane of the objective lens in a Transmission Electron Microscope. Use of precisely positioned phase plates can increase resolution in electron tomography, and also increase throughput when using the single-particle approach. Our specific aim is to provide a phase plate system that will include a modified objective apert ure holder that incorporates (1) a piezo positioning system, (2) a heater to avoid contamination, and (3) an electrical feed-through for use with electrostatic phase plates. Simply exchanging the original objective aperture holder with the Hummingbird phas e plate holder will allow nearly any cryo-EM to be equipped with a phase plate. The cost of this holder will be substantially lower than any other technology that can yield a similar improvement in imaging of frozen-hydrated specimens. In close collaborati on with the Resource for the Visualization of Biological Complexity (RVBC) at the Wadsworth Center's NIH Biotechnological Resource, we will develop a new phase plate holder, demonstrate its use, and publish the results. It is our intent to develop and comm ercialize a cost effective system that will enable these techniques to become widely available to the biology research community. This proposal will develop tools that allow biologists to get a clearer picture of the structure and function of the cell. Thi s knowledge is fundamental to innumerable areas of medical research and practice.

* information listed above is at the time of submission.

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