Phase plate holder for transmission electron microscopy

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43GM085841-01A1
Agency Tracking Number: GM085841
Amount: $100,000.00
Phase: Phase I
Program: SBIR
Awards Year: 2009
Solicitation Year: 2009
Solicitation Topic Code: N/A
Solicitation Number: PHS2009-2
Small Business Information
DUNS: 171838019
HUBZone Owned: Y
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 (360) 252-2737
Business Contact
Phone: (360) 252-2737
Research Institution
DESCRIPTION (provided by applicant): The objective of our application is to develop hardware and procedures that will allow researchers to practically utilize phase plates in the back focal plane of the objective lens in a Transmission Electron Microscope. Use of precisely positioned phase plates can increase resolution in electron tomography, and also increase throughput when using the single-particle approach. Our specific aim is to provide a phase plate system that will include a modified objective aperture holder that incorporates (1) a piezo positioning system, (2) a heater to avoid contamination, and (3) an electrical feed-through for use with electrostatic phase plates. Simply exchanging the original objective aperture holder with the Hummingbird phase plate holder will allow nearly any cryo-EM to be equipped with a phase plate. The cost of this holder will be substantially lower than any other technology that can yield a similar improvement in imaging of frozen-hydrated specimens. In close collaboration with the Resource for the Visualization of Biological Complexity (RVBC) at the Wadsworth Center's NIH Biotechnological Resource, we will develop a new phase plate holder, demonstrate its use, and publish the results. It is our intent to develop and commercialize a cost effective system that will enable these techniques to become widely available to the biology research community. This proposal will develop tools that allow biologists to get a clearer picture of the structure and function of the cell. This knowledge is fundamental to innumerable areas of medical research and practice.

* Information listed above is at the time of submission. *

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