Site-specific Recombinases For Use In Hybrid

Award Information
Agency:
Department of Agriculture
Branch
n/a
Amount:
$55,000.00
Award Year:
1997
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
37694
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Hybrigene
Purdue Research Park, Business & Technology Div., West Lafayette, IN, 47906
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
DR. HONG LUO
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
The objective of this project is to evaluate the efficacy of the FLP/FRT site-specific recombinase inexcising a transgenic tapetum-specific promoter driving an inverted (antisense) Arabidopsis fertilitygene, bcl1. Plants containing the antisense of bcl1 (flanked by FRT sites) will be male sterile and whencrossed to plants containing the FLP site-specific recombinase, FLP should excise the sterility gene thusrestoring fertility and producing a hybrid. In addition, the relative effectiveness of different structuralalterations in the FRT sites will be assessed with respect to the efficiency of transgene excisions. If thisFLP/FRT system functions at sufficient efficiencies, it will confirm the feasibility that it can be used asa new method for making hybrid plants.During Phase II of this SBIR, it will be our intention to use the same system, if it proves to be feasibleduring Phase I research, for the development of corn hybrids.

* information listed above is at the time of submission.

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