THE FORMATION OF DNA-CARCINOGEN ADDUCTS IS AN IMPORTANT ENVIRONMENTAL FACTOR IN CARCINOGENESIS.

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 3057
Amount: $45,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1985
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Imbic Corp.
P.o. Box 7539, Columbia, MO, 65205
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 JOHN E. MCENTIRE
 PRINCIPAL INVESTIGATOR
 (314) 874-2481
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE FORMATION OF DNA-CARCINOGEN ADDUCTS IS AN IMPORTANT ENVIRONMENTAL FACTOR IN CARCINOGENESIS. RECENT TECHNIQUES FOR ISOLATION AND CHARACTERIZATION OF DEOXYNUCLEOSIDE-CARCINOGEN ADDUCTS AND SUBSEQUENT IMMUNOASSAY PROCEDURES CAN EFFECTIVELY DETECT THESE ADDUCTS FROM CELL LYSATES IN THE RANGE OF 1 ADDUCT/107 BASES. WE WILL USE OUR COMBINED TECHNICAL EXPERTISE IN THE AREAS OF NUCLEOTIDE/NUCLEOSIDE CHEMISTRY AND CHROMATOGRAPHY, AND IMMUNOLOGY IN A PHASE I STUDY FOR PRODUCTION OF MONOCLONAL ANTIBODIES TO ACROLEIN AND 1-NITROPYRENE ADDUCTS TO DEOXYNUCLEOSIDES AS THE MODEL SYSTEMS. AFTER DEMONSTRATION OF FEASIBILITY OF THE TECHNOLOGY AND COMPLETION OF THE PHASE I OBJECTIVES, PHASE II STUDIES WILL USE THE EXPERIMENTAL PROTOCOLS AND PHASE I EXPERIENCE TO INVESTIGATE ADDITIONAL CARCINOGENS AND MINOR ADDUCTS LISTED IN THE REQUEST. ADDUCTS FORMED WITH RIBONUCLEOSIDES WHICH MAY HAVE HIGHER TURNOVER RATES WILL ALSO BE STUDIED IN PHASE II. THE LONG-TERM OBJECTIVE OF THE PROJECT IS TO DEVELOP NONINVASIVE, ACCURATE, ECONOMICAL, SENSITIVE, AND SELECTIVE IMMUNOASSAY TEST KITS FOR MONITORING PERSONNEL EXPOSURE TO CARCINOGENS.

* information listed above is at the time of submission.

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