RAPID DETECTION SYSTEM FOR HTLV-III

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 7089
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1987
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Imclone Systems Inc
180 Varick Street, New York, NY, 10014
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 NICHOLAS GIORGIO PHD
 (212) 645-1405
Business Contact
Phone: () -
Research Institution
N/A
Abstract
IMCLONE SYSTEMS, INC. SEEKS TO DEVELOP A NOVEL RAPID DETECTION SYSTEM FOR THE IDENTIFICATION OF ANTIBODIES DIRECTED AGAINST HUMAN T-CELL LYMPHOTROPHIC VIRUS III (HTLV-III). THE SYSTEM IS BASED ON A SANDWICH FLUORO-IMMUNOASSAY IN WHICH AN HTLV-III ANTIBODY CONTAINING SERUM SAMPLE IS PREMIXED WITH FLUORESCEINATED HTLV-III VIRAL ANTIGEN CONJUGATE AND SUBSEQUENTLY ALLOWED TO INCUBATEWITH AN OPTICAL GRADE FIBER TO WHICH HTLV-III VIRAL ANTIGENSHAVE BEEN COVALENTLY ATTACHED. ACCUMULATION OF THE FLUORESCEINED HTLV-III VIRAL ANTIGENS ONTHE FIBER SURFACE PROVIDES A LOCALIZED CONCENTRATION OF FLUORESCEIN WHICH IS EXCITED BY ELECTROMAGNETIC RADIATION IN THE VISIBLE WAVELENGTH RANGE OF 490 NM. EMISSION OF FLUORESCEIN AT 525 NM IS RECAPTURED BY THE FIBER AND PROPAGATED TO THE END WHERE IT IS COLLECTED AND DETECTED AS A LIGHT SIGNAL OUTPUT. THIS SYSTEM HAS THE POTENTIAL TO BECOME A SENSITIVE, RAPID DETECTION ASSAY WITH SIMPLIFIEDSAMPLE HANDLING, SMALL SAMPLE REQUIREMENTS, AND WIDE USE IN NUMEROUS CLINICAL SETTINGS INCLUDING PHYSICIANS' OFFICES, CLINICAL LABORATORIES, AND BLOOD BANKS.

* information listed above is at the time of submission.

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