THE LONG-TERM OBJECTIVE OF THIS PROJECT IS THE MOLECULAR CHARACTERIZATION (CDNA CLONING, EXPRESSION) OF MURINE AND HUMAN GENES CODING FOR A GROWTH FACTOR FOR MATURE B- LYMPHOCYTES (M-BC-GF).

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 4692
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1986
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Immunex Corp
51 University Street, Seattle, WA, 98101
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 CHRISTOPHER S HENNEY
 PRINCIPAL INVESTIGATOR
 (206) 587-0430
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE LONG-TERM OBJECTIVE OF THIS PROJECT IS THE MOLECULAR CHARACTERIZATION (CDNA CLONING, EXPRESSION) OF MURINE AND HUMAN GENES CODING FOR A GROWTH FACTOR FOR MATURE B- LYMPHOCYTES (M-BC-GF). OUR APPROACH WILL BE TO CONSTRUCT A CDNA LIBRARY FROM SIZED, ACTIVE, M-BC-GF MRNA AND TO ISOLATEA FULL-LENGTH M-BD-GF CDNA BY HYBRIDIZATION WITH AN OLIGONUCLEOTIDE PROBE DEDUCED FROM PROTEIN SEQUENCE INFORMATION DERIVED FROM PURIFIED M-BC-GF PROTEIN. DURING PHASE I, M-BC-GF ACTIVITY WILL BE PURIFIED FROM SUPERNATES PRODUCED BY A CLONED MURINE LYMPHOMA CELL LINE (EL-4 LX CELLLINE) RECENTLY DEVELOPED IN OUR LABORATORY. MRNA CODING FORM-BC-GF ACTIVITY IN VITRO WILL ALSO BE PREPARED FROM THE EL-4 LX CELL LINE AND SIZE FRACTIONATED IN PREPARATION FOR CDNA CLONING. ONCE MURINE M-BC-GF CDNAS ARE ISOLATED, THEY WILL BE EXPRESSED IN APPROPRIATE YEAST AND E. COLI VECTORS. RECOMBINANT M-BC-GF WILL THEN BE TESTED FOR BIOLOGICAL ACTIVITY IN MURINE MODEL SYSTEMS OF B-CELL DEFICIENCY. FINALLY, MURINE CDNAS FOR M-BC-GF WILL BE USED AS PROBES FORIDENTIFICATION BY SOUTHERN BLOT HYBRIDIZATION OF ANALOGOUS HUMAN CDNAS. M-BC-GF COULD BE OF VALUE IN CLINICAL RESTORATION OF ANTIBODY RESPONSIVENESS IN CASES OF VIRAL, CHEMOTHERAPEUTIC, OR RADIATION INDUCED IMMUNE SUPPRESSION, AND FOR THE IN VITRO GENERATION OF HUMAN B-LYMPHOCYTES AND/ OR HYBRIDOMAS.

* information listed above is at the time of submission.

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