THE LONG-TERM OBJECTIVE OF THIS PROJECT IS THE MOLECULAR CHARACTERIZATION (CDNA CLONING, EXPRESSION) OF MURINE AND HUMAN GENES CODING FOR A GROWTH FACTOR FOR PREMATURE B- LYMPHOCYTES (PRE-BC-GF).

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 4693
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1986
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
51 University Street, Seattle, WA, 98101
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 STEVEN GILLIS
 PRINCIPAL INVESTIGATOR
 (206) 587-0430
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE LONG-TERM OBJECTIVE OF THIS PROJECT IS THE MOLECULAR CHARACTERIZATION (CDNA CLONING, EXPRESSION) OF MURINE AND HUMAN GENES CODING FOR A GROWTH FACTOR FOR PREMATURE B- LYMPHOCYTES (PRE-BC-GF). THE APPROACH WILL BE TO CONSTRUCT A CDNA LIBRARY FROM SIZED, ACTIVE, PRE-BC-GF MRNA AND TO ISOLATE A FULL-LENGTH PRE-BC-GF CDNA BY HYBRIDIZATION WITH AN OLIGONUCLEOTIDE PROBE DEDUCED FROM PROTEIN SEQUENCE INFORMATION DERIVED FROM PURIFIED PRE-BC-GF PROTEIN. DURING PHASE I, PRE-BC-GF ACTIVITY WILL BE PURIFIED FROM SUPERNATES PRODUCED BY A TRANSFECTED/TRANSFORMED MURINE STROMAL BONE MARROW CELL LINE (SV CELL LINE) RECENTLY DEVELOPED IN OUR LABORATORY. THE MRNA CODING FOR PRE-BC-GF ACTIVITY IN VITRO WILL ALSO BE PREPARED FROM THE SV CELL LINE AND SIZE FRACTIONATED IN PREPARATION FOR CDNA CLONING. ONCE MURING PRE-BC-GF CDNAS ARE ISOLATED, THEY WILL BE EXPRESSED IN APPROPRIATE YEAST AND E. COLI VECTORS. RECOMBINANT PRE-BC-GF WILL THEN BE TESTED FOR BIOLOGICAL ACTIVITY IN MURINE MODEL SYSTEMS OF B-CELL DEFICIENCY. FINALLY, MURINE CDNAS FOR PRE-BC-GF WILL BE USED AS PROBES FOR IDENTIFICATION BY SOUTHERN BLOT HYBRIDIZATION OF ANALOGOUS HUMAN CDNAS. PRE-BC-GF COULD BE OF VALUE IN CLINICAL RESTORATION OF ANTIBODY RESPONSIVENESS IN CASES OF VIRAL, CHEMOTHERAPEUTIC, OR RADIATION INDUCED IMMUNE SUPPRESSION.

* Information listed above is at the time of submission. *

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