THE OBJECTIVE OF THIS PHASE I PROPOSAL IS TO DEMONSTRATE THAT CULTURED HUMAN COLON TUMOR CELL LINES PRODUCE AUTO-INHIBITORY FACTORS.

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$48,300.00
Award Year:
1984
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
1402
Agency Tracking Number:
1402
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
1000 Seneca Street, Seattle, WA, 98101
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
JOHN C. HOUCK
PRESIDENT
() -
Business Contact:
() -
Research Institute:
n/a
Abstract
THE OBJECTIVE OF THIS PHASE I PROPOSAL IS TO DEMONSTRATE THAT CULTURED HUMAN COLON TUMOR CELL LINES PRODUCE AUTO-INHIBITORY FACTORS. IN ADDITION, THE MOLECULAR WEIGHT OF THE INHIBITORY FACTOR WILL BE DETERMINED. CONDITIONED MEDIA WILL BE COLLECTED FROM THE SW-480 CELL LINE UNDER BOTH SERUM-CONTAINING AND SERUM-FREE CONDITIONS. THE FRACTIONATION PROCEDURES HAVE BEEN DESIGNED TO EFFECTIVELY IDENTIFY BOTH POSSIBLE HIGH MOLECULAR WEIGHT AND LOW MOLECULAR WEIGHT INHIBITORY FACTORS. THE SPECIFICITY OF THE INHIBITOR WILL BE DETERMINED BY IN VITRO ASSAYS WITH A WIDE VARIETY OF NORMAL AND TUMOR CELL LINES. THE SUCCESSFUL COMPLETION OF PHASE I WILL PROVIDE THE DATA NECESSARY FOR FURTHER DEVELOPMENT OF AUTO-INHIBITORY FACTORS FOR HUMAN COLON TUMORS. THE MOLECULAR WEIGHT DETERMINED DURING PHASE I WILL BE USED TO DESIGN THE NECESSARY PROTOCOLS FOR COMPLETE PURIFICATION OF THE INHIBITORY FACTOR AND ITS EVENTUAL LARGE SCALE PRODUCTION. THIS PRODUCTION WILL BE THROUGH EITHER GENE-CLONING IF THE INHIBITOR IS OF HIGH MOLECULAR WEIGHT OR BY CHEMICAL SYNTHESIS IF THE INHIBITOR IS LOW MOLECULAR WEIGHT. THE SUCCESSFUL DEVELOPMENT OF SUCH AN INHIBITOR, SPECIFIC FOR COLON TUMORS, WOULD BE OF GREAT USEFULNESS IN THE TREATMENT OF COLON CANCER, ONE OF THE PREDOMINANT HUMAN CANCERS.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government