RAPID DETECTION OF ESCHERICHIA COLI 0157:H7

Award Information
Agency: Department of Agriculture
Branch: N/A
Contract: N/A
Agency Tracking Number: 41913
Amount: $65,000.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 1998
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
717 Yosemite Circle, Denver, CO, 80220
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Dr Robert T McCalmon
 Principal Investigator
 () -
Business Contact
Phone: () -
Research Institution
N/A
Abstract
Escherichia coli 0157:H7 is an emerging cause of foodborne illness first identified as an etiological agent in 1982. There is a need for low complexity, rapid (less than four hours) detection method capable of being performed on site at food processing plants in association with Hazard Analysis and Critical Control Plans (HACCP). Ideally, this test should be simple to perform and interpret, be low cost, and be a sensitive and specific method for rapid detection of pathogenic E. coli 0157:H7. Methods will be developed for the detection of viable E. coli 0157:H7 contamination of meat carcass or ground beef. This consists of specimen collection from these materials, extraction and capture of 0157:H7 RNA, isothermal amplification, and visual lateral flow detection. The final assay will be incorporated into a self contained integrated particle (SCIP) device that is easy to use, does not require expensive instrumentation, and can readily be performed on site.

* Information listed above is at the time of submission. *

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