A Multiplexed Point-of-Care Assay for the Detection of Enteric Pathogens that Cause Severe Diarrhea in Deployed Soldiers

Award Information
Agency: Department of Defense
Branch: Army
Contract: W81XWH-07-C-0030
Agency Tracking Number: A062-158-0254
Amount: $119,587.00
Phase: Phase I
Program: SBIR
Awards Year: 2006
Solicitation Year: 2006
Solicitation Topic Code: A06-158
Solicitation Number: 2006.2
Small Business Information
562 Ist Ave S, Suite 600, Seattle, WA, 98104
DUNS: 004006628
HUBZone Owned: N
Woman Owned: Y
Socially and Economically Disadvantaged: N
Principal Investigator
 William John Woodroofe MORROW
 Principal Investigator
 (206) 344-5821
 wjwmorrow@inbios.com
Business Contact
 Estela FIGUEROA
Title: President
Phone: (206) 344-5821
Email: inbios@aol.com
Research Institution
N/A
Abstract
The objective of this study is to develop a robust, field-grade, screening assay for the detection of severe diarrheal disease-causing pathogens including E. coli O157, Salmonella spp., Shigella spp., Cryptosporidium spp., Campylobacter spp., Noroviruses, and Rotaviruses. The test will utilize lateral flow (dipstick) chromatography incorporated into a unique, multiplex cassette that enables the simultaneous screening of multiple analytes. The proposed immunochromatographic assay would be a very useful rapid screening test to assess individuals presenting with diarrhea of likely infectious etiology. In this Phase 1 study we shall evaluate the ability of a panel of antibodies, with specificity for the families of enteric pathogens listed above, to function as the basis of an antigen-capture detection system. We will then construct prototype assays that will be evaluated against samples from individuals with confirmed infections. Further development of the assay would take place in a Phase 2 proposal.

* Information listed above is at the time of submission. *

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