A Point-of-Care Assay for the Detection of Spotted-fever group and Typhus group Rickettsia.

Award Information
Agency: Department of Defense
Branch: Army
Contract: W81XWH-08-C-0006
Agency Tracking Number: A072-153-0025
Amount: $119,986.00
Phase: Phase I
Program: SBIR
Awards Year: 2007
Solicitation Year: 2007
Solicitation Topic Code: A07-153
Solicitation Number: 2007.2
Small Business Information
INBIOS INTERNATIONAL
562 Ist Ave S, Suite 600, Seattle, WA, 98104
DUNS: 004006628
HUBZone Owned: N
Woman Owned: Y
Socially and Economically Disadvantaged: N
Principal Investigator
 William John Woodroofe MORROW
 Director of New Technology
 (206) 344-5821
 wjwmorrow@inbios.com
Business Contact
 Estela Raychaudhuri
Title: President
Phone: (206) 344-5821
Email: inbios@aol.com
Research Institution
N/A
Abstract
The objective of this study is to develop a robust, field-grade, screening assay for the differential diagnosis of infection by Spotted Fevers (e.g. Rocky Mountain Spotted fever, Boutonneuse fever) and Typhus (Epidemic and Endemic forms) causing rickettsial bacteria. Scrub Typhus (Orientia tsutsugamushi) will be used as a reference control and may be incorporated into the final assay. The test will utilize lateral flow (dipstick) chromatography incorporated into a unique, triplex, cassette that enables the simultaneous screening of Spotted Fever, Typhus and Scrub Typhus group organisms. The proposed immunochromatographic assay would be a very useful for point-of-care screening and as differential diagnostic test to assess individuals presenting with symptoms of infection. In this Phase 1 study we shall evaluate the ability of a panel of rickettsia-derived antigens from the families of pathogens listed above to function as the basis of an IgM/IgG serodetection system. We will then construct prototype assays that will be evaluated against samples from individuals with confirmed infections. Further development of the assay would take place in a Phase 2 study.

* information listed above is at the time of submission.

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