A Multiplexed Point-of-Care Assay for the Detection of Enteric Pathogens that Cause Severe Diarrhea in Deployed Soldiers

Award Information
Agency:
Department of Defense
Branch
Army
Amount:
$724,941.00
Award Year:
2008
Program:
SBIR
Phase:
Phase II
Contract:
W81XWH-07-C-0030
Award Id:
77443
Agency Tracking Number:
A062-158-0254
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
562 Ist Ave S, Suite 600, Seattle, WA, 98104
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
004006628
Principal Investigator:
W. Morrow
PI
(206) 344-5821
wjwmorrow@inbios.com
Business Contact:
Estela Raychaudhuri
President
(206) 344-5821
inbios@aol.com
Research Institution:
n/a
Abstract
The objective of this study is to develop a robust, economical, field-grade, screening assay for the detection of severe diarrhea-causing pathogens including Salmonella spp., E. coli O157, Shigella spp., Cryptosporidium spp., Campylobacter spp., Noroviruses and Rotaviruses. The test will utilize lateral flow (dipstick) chromatography incorporated into a unique, multiplex cassette that will enable the simultaneous screening of multiple analytes. The proposed immunochromatographic assay would be a very useful rapid screening test to assess individuals presenting with diarrhea of likely infectious etiology. In this Phase II study we will continue the preliminary development work started in the Phase I feasibility investigation. Specifically we will: (1) continue to identify produce, evaluate and optimize the target antigens from the above panel of pathogens in lateral flow systems; (2) as necessary, identify complementary target antigens for each disease category for added sensitivity and specificity; (3) design and development sample preparatory and lateral flow platforms for each assay and combine into a multiplex device; and (5) evaluate the clinical sensitivity and specificity of the test using fecal specimens from confirmed infected, or control individuals.

* information listed above is at the time of submission.

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