A Point-of-Care Assay for the Detection of Spotted-fever group and Typhus group Rickettsia.

Award Information
Agency: Department of Defense
Branch: Army
Contract: W81XWH-08-C-0006
Agency Tracking Number: A072-153-0025
Amount: $1,209,590.00
Phase: Phase II
Program: SBIR
Awards Year: 2008
Solicitation Year: 2007
Solicitation Topic Code: A07-153
Solicitation Number: 2007.2
Small Business Information
INBIOS INTERNATIONAL
562 1st Avenue South, Suite 600, Seattle, WA, 98104
DUNS: 004006628
HUBZone Owned: N
Woman Owned: Y
Socially and Economically Disadvantaged: N
Principal Investigator
 John Morrow
 Director New Technology A
 (206) 344-5821
 wjwmorrow@inbios.com
Business Contact
 Tatiana Leshchinsky
Title: Assistant Project Manager
Phone: (206) 344-5821
Email: Tatiana@inbios.com
Research Institution
N/A
Abstract
The objective of this study is to develop a robust, field-grade, screening assay for the differential diagnosis of infection by Spotted Fevers (e.g. Rocky Mountain Spotted fever, Boutonneuse fever) and Typhus (Epidemic and Endemic forms) causing rickettsial bacteria. Scrub Typhus (Orientia tsutsugamushi) will be used as a reference control and may be incorporated into the final assay. In this Phase II component we will build on our Phase I studies and continue to identify antigens that will enable rapid detection of rickettsial infection with a high degree of sensitivity and specificity. We intend to accomplish these objectives using a bioinformatics and a novel proteomic display technique developed at InBios. We will also make use of a network of international collaborators who will provide reagents as well as sera from rickettsia-infected individuals as well as animals. The test will utilize lateral flow (dipstick) chromatography incorporated into a unique cassette that will enable the simultaneous screening of Spotted Fever, Typhus (and possibly Scrub Typhus) group organisms. The proposed immunochromatographic assay will be very useful for point-of-care screening and as differential diagnostic test to assess infection in deployed military and other personnel presenting with symptoms of infection.

* information listed above is at the time of submission.

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