Analysis of tracking based phenotypes in CNS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$1,365,115.00
Award Year:
2009
Program:
SBIR
Phase:
Phase II
Contract:
2R44GM077774-02
Award Id:
79848
Agency Tracking Number:
GM077774
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
DRVISION TECHNOLOGIES, LLC, 15921 NE 8TH ST, STE 200, BELLEVUE, WA, -
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
827582656
Principal Investigator:
SHIHJONG LEE
(425) 653-5589
JAMESL@DRVTECHNOLOGIES.COM
Business Contact:
SAMUEL ALWORTH
() -
Research Institution:
n/a
Abstract
DESCRIPTION (provided by applicant): Project Summary The objective of this project is to develop Prototype and Scale kinetic image recognition software to score tracking based phenotypes in a broad range of microscopy based central nervous system (CNS) a pplications. The target software tool can be flexibly taught to score or screen the precise dynamic phenotypes of interest, and scalable, together with the imaging assay platform (e.g. imaging system, reagents, etc.) to provide high throughput scoring. It will provide 1) motion signal decomposition to isolate heterogeneous spatial-temporal patterns; 2) teachable subcellular object detection; 3) teachable subcellular object tracking; 4) comprehensive track characterization; and 5) kinetic data analysis inter faces to support dynamic phenotype discovery and scoring. We propose to expand and commercialize our tracking technologies including teachable tracking state based tracking, motion energy tracking enhancement and track classification. The work product will be incorporated into our flagship product SVCell, and sold through our commercial partners including Nikon Corporation. The hypothesis is that our tracking software can be used for the accurate and robust analysis of a broad range of CNS applications, and scalable for the high throughput scoring of dynamic tracking characteristics with high speed, accuracy and reliability. To test this hypothesis, we set forth the following specific aims: Aim 1: Optimize the teachable tracking module for a broad range of t racking based phenotypes; Aim 2: Optimize and validate the high throughput scoring of tracking based phenotypes; Aim 3: Evaluate the product readiness of the SVCell tracking beta through field tests and scientific collaborations. This project is significan t because technologies are needed which can flexibly screen a variety of tracking based, dynamic phenotypes at high throughput to support practical and effective CNS related scientific programs in basic research (e.g. phenotyping) and applied research (e.g . drug discovery). Analysis of these dynamic phenotypes in time-lapse microscopy movies could provide insights into CNS disease formation and enable earlier and more effective interventions. PUBLIC HEALTH RELEVANCE: Project Narrative Time-lapse microscopy image recognition can make a significant impact on the fight against neurological diseases and mental illness. By quantifying complex, dynamic phenotypes in the movies, it gives scientists a powerful tool with which to discover the basic mechanisms underly ing important CNS functions, and then screen methods that act on them. This could enable earlier and more effective therapeutic interventions.

* information listed above is at the time of submission.

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