Enhancing site-specific chromosomal integration in clostridia

Award Information
Agency:
Department of Energy
Branch
n/a
Amount:
$99,682.00
Award Year:
2009
Program:
SBIR
Phase:
Phase I
Contract:
DE-FG02-09ER85341
Award Id:
94448
Agency Tracking Number:
91201
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Elcriton (Currently ELCRITON, INC)
15 Innovation Way, #288, Newark, DE, 19711
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
828815477
Principal Investigator:
Bryan Tracy
Mr.
(864) 921-5146
bryantracy2009@u.northwestern.edu
Business Contact:
Danielle Nolen
Ms.
(302) 528-3205
dnolen68@gmail.com
Research Institution:
n/a
Abstract
The bacteria genus, Clostridium, includes many species that are naturally capable of degrading cellulose and producing valuable chemicals and fuel alcohols. Unfortunately the genetic engineering tools for developing improved strains are limited for all clostridia. This project will develop novel approaches for enhancing chromosomal integration in all clostridia species. With an enhanced recombination system for clostridia, it would become possible to quickly knock out and knock in genes, allowing new strains with superior production capabilities to be quickly engineered. Phase I will demonstrate the feasibility of improving site-specific chromosomal integration via recombinant over-expression of native and heterologous proteins involved in homologous recombination. Phase I will focus on the over-expression of individual proteins, and Phase II will build upon those results by studying the over-expression of combinations of proteins. Commercial Applications and other Benefits as described by the awardee The technology should significantly accelerate strain development in solventogenic and other clostridia species, leading to the microbial conversion of renewable carbohydrates to chemicals and fuels. Such a development would aid our nation¿s goals of developing sustainable and greener technologies for the production of chemicals and transportation fuels

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government