Rodent Cell Line Authentication

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R41GM086896-01
Agency Tracking Number: GM086896
Amount: $99,999.00
Phase: Phase I
Program: STTR
Awards Year: 2009
Solicitation Year: 2009
Solicitation Topic Code: N/A
Solicitation Number: PHS2009-2
Small Business Information
IMPACT BIOLABS, LLC
IMPACT BIOLABS, LLC, 4011 Discovery Drive, COLUMBIA, MO, 65201
DUNS: 608611302
HUBZone Owned: Y
Woman Owned: Y
Socially and Economically Disadvantaged: N
Principal Investigator
 ELIZABETH BRYDA
 (573) 882-5504
 BRYDAE@MISSOURI.EDU
Business Contact
 RILEY LELA
Phone: (573) 356-1896
Email: contact@impactbiolabs.com
Research Institution
 UNIVERSITY OF MISSOURI
 UNIVERSITY OF MISSOURI
UNIVERSITY HALL
COLUMBIA, MO, 65211 1508
 Nonprofit college or university
Abstract
DESCRIPTION (provided by applicant): The long term goal of this research is to develop an efficient method to authenticate rodent cell lines. Misidentification as well as inter- and intra-species cross contamination of cell lines is widespread and has a substantial negative impact on biomedical research. The use of short tandem repeat (STR) analysis has been shown to be an effective means of authenticating human cell lines, STR genetic profiles have been established for a large number of human cell lines, and much of this data is publicly accessible in the ATCC STR Profile Database. Equivalent resources do not currently exist for authentication of rodent cell lines. In order to fill this gap, the specific aims of this application are to develop a multiplex microsatellite marker-based test to generate unique genetic profiles for mouse and rat cell lines and to build a database of these profiles to use for rodent cell line authentication. By developing these resources, it will be possible to provide a cost-effective and accurate method for rodent cell line authentication for the research community. PUBLIC HEALTH RELEVANCE: The use of cell lines is a cornerstone of basic biomedical research and the ability to ensure the authenticity of the cell lines is critical to ensuring the integrity and validity of the research results that guide scientific advances affecting public health. Establishing a method for generating unique genetic profiles for rodent cell lines provides a quick, sensitive, and accurate means to verify the authenticity of those cell lines being used in research laboratories.

* information listed above is at the time of submission.

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