Rodent Cell Line Authentication

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$99,999.00
Award Year:
2009
Program:
STTR
Phase:
Phase I
Contract:
1R41GM086896-01
Award Id:
93808
Agency Tracking Number:
GM086896
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
IMPACT BIOLABS, LLC, 4011 Discovery Drive, COLUMBIA, MO, 65201
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
608611302
Principal Investigator:
ELIZABETHBRYDA
(573) 882-5504
BRYDAE@MISSOURI.EDU
Business Contact:
RILEYLELA
() -
contact@impactbiolabs.com
Research Institute:
UNIVERSITY OF MISSOURI

UNIVERSITY OF MISSOURI
UNIVERSITY HALL
COLUMBIA, MO, 65211 1508

Nonprofit college or university
Abstract
DESCRIPTION (provided by applicant): The long term goal of this research is to develop an efficient method to authenticate rodent cell lines. Misidentification as well as inter- and intra-species cross contamination of cell lines is widespread and has a su bstantial negative impact on biomedical research. The use of short tandem repeat (STR) analysis has been shown to be an effective means of authenticating human cell lines, STR genetic profiles have been established for a large number of human cell lines, a nd much of this data is publicly accessible in the ATCC STR Profile Database. Equivalent resources do not currently exist for authentication of rodent cell lines. In order to fill this gap, the specific aims of this application are to develop a multiplex m icrosatellite marker-based test to generate unique genetic profiles for mouse and rat cell lines and to build a database of these profiles to use for rodent cell line authentication. By developing these resources, it will be possible to provide a cost-effe ctive and accurate method for rodent cell line authentication for the research community. PUBLIC HEALTH RELEVANCE: The use of cell lines is a cornerstone of basic biomedical research and the ability to ensure the authenticity of the cell lines is critical to ensuring the integrity and validity of the research results that guide scientific advances affecting public health. Establishing a method for generating unique genetic profiles for rodent cell lines provides a quick, sensitive, and accurate means to ver ify the authenticity of those cell lines being used in research laboratories.

* information listed above is at the time of submission.

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