RAPID, SINGLE MOLECULAR DNA SEQUENCING NANOPORE DEVICE

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$1,207,904.00
Award Year:
2002
Program:
SBIR
Phase:
Phase II
Contract:
2R44HG002327-02
Agency Tracking Number:
HG002327
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
ADVANCED RESEARCH CORPORATION
ADVANCED RESEARCH CORPORATION, 815 14TH AVE SE, MINNEAPOLIS, MN, 55414
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
MATTHEW DUGAS
(612) 331-2211
mdugas@gotoarc.com
Business Contact:
JUDY DUGAS
(612) 331-2211
JDUGAS@GOTOARC.COM
Research Institution:
n/a
Abstract
DESCRIPTION (provided by applicant): The objective of this Phase II program is to complete the development of a commercializable solid state nanopore device for the accurate high speed sequencing of DNA. To achieve this goal a manufacturable device must be designed and fabrication processes established that result in a unit with extremely small, well controlled diameter orifices of approximately 2 nm through a multi-layered thin film sandwich of insulators and conductors. The Phase I portion of this program established the methodology of: creating a thin (30 nm) SiN film over a 20 um square window in a 1 cm square silicon frame; Focused Ion Beam (FIB) milling a nanopore; subsequent deposition of a multi-layer thinfilm sandwich of conductor and insulating layers on the surface of SiN film; and building a cartridge to hold and seal the nanopore chip between two liquid cells, and provide electrical terminations for measuring the changes in current, potential, or capacitance resulting in the passage of a DNA molecule chain through the nanopore. It was found that FIB milling produced a nanopore that was too wide and lacked the required sensitivity to map DNA molecules passing through the nanopore.. A new approach, TEM drilling will be used to achieve nanopores of 2 nm or smaller. Additional work will be invested in optimizing the leads and instrumentation, i.e., patch clamp amplifiers to achieve improved signal-to-noise ratios and increased detection sensitivity.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government