RAPID, SINGLE MOLECULAR DNA SEQUENCING NANOPORE DEVICE

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 2R44HG002327-02
Agency Tracking Number: HG002327
Amount: $1,207,904.00
Phase: Phase II
Program: SBIR
Awards Year: 2002
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
ADVANCED RESEARCH CORPORATION
ADVANCED RESEARCH CORPORATION, 815 14TH AVE SE, MINNEAPOLIS, MN, 55414
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 MATTHEW DUGAS
 (612) 331-2211
 mdugas@gotoarc.com
Business Contact
 JUDY DUGAS
Phone: (612) 331-2211
Email: JDUGAS@GOTOARC.COM
Research Institution
N/A
Abstract
DESCRIPTION (provided by applicant): The objective of this Phase II program is to complete the development of a commercializable solid state nanopore device for the accurate high speed sequencing of DNA. To achieve this goal a manufacturable device must be designed and fabrication processes established that result in a unit with extremely small, well controlled diameter orifices of approximately 2 nm through a multi-layered thin film sandwich of insulators and conductors. The Phase I portion of this program established the methodology of: creating a thin (30 nm) SiN film over a 20 um square window in a 1 cm square silicon frame; Focused Ion Beam (FIB) milling a nanopore; subsequent deposition of a multi-layer thinfilm sandwich of conductor and insulating layers on the surface of SiN film; and building a cartridge to hold and seal the nanopore chip between two liquid cells, and provide electrical terminations for measuring the changes in current, potential, or capacitance resulting in the passage of a DNA molecule chain through the nanopore. It was found that FIB milling produced a nanopore that was too wide and lacked the required sensitivity to map DNA molecules passing through the nanopore.. A new approach, TEM drilling will be used to achieve nanopores of 2 nm or smaller. Additional work will be invested in optimizing the leads and instrumentation, i.e., patch clamp amplifiers to achieve improved signal-to-noise ratios and increased detection sensitivity.

* information listed above is at the time of submission.

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