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Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 75N95020C00018
Agency Tracking Number: 75N95020C00018-0-9999-0
Amount: $225,000.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: 19
Solicitation Number: N/A
Solicitation Year: 2019
Award Year: 2020
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
ASHLAND, MA 01721-1717
United States
DUNS: 147365936
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Mitchell Klausner
 (508) 881-6771
Business Contact
 Mitchell Klausner
Phone: (508) 881-6771
Research Institution

The need for more complex 3D tissue models by pharmaceutical and academic researchers is increasing. A key factor for certain tissue models is a scaffold that promotes structural integrity, cell anchorage, viability and proliferation but which over time degrades (so that it can be replaced by naturally produced ECM) and allows cell-to-cell interaction in co-culture models. No high throughput, cell culture insert plates (CCIP) are currently available with biodegradable membranes. In the current proposal, we will produce a 96-well CCIP with a biodegradable membrane. During Phase 1, a biocompatible, electrospun membrane of the prescribed biodegradation rate, thickness, and pore size will be identified. Initially, we will test biocompatible adhesives and develop techniques to attach the membrane to single-well cell culture inserts (CCI) along with a ridge that will be attached to the CCI underside. CCI biocompatibility will be verified by culturing a highly differentiated, 3D tissue model for ≥30 days. The techniques to adhere the membrane and ridge will be adapted to produce a prototype 96-well CCIP and its properties will be verified. In Phase 2, molds for the CCIP and accompanying plates will be made, and membrane and ridge adhering manufacturing techniques will be refined to allow commercial production of 96-well CCIP.

* Information listed above is at the time of submission. *

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