RAPID DIAGNOSTIC TEST FOR HUMAN FUNGAL PATHOGENS

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 4694
Amount: $500,000.00
Phase: Phase II
Program: SBIR
Awards Year: 1988
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Integrated Genetics Inc.
31 New York Avenue, Framingham, MA, 01701
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Jeffrey F Lemontt
 Principal Investigator
 (617) 875-1336
Business Contact
Phone: () -
Research Institution
N/A
Abstract
LOCALIZED AND SYSTEMIC MYCOSES CAUSED BY PATHOGENIC FUNGI (E.G., THE YEASTS CANDIDA ALBICANS AND CRYPTOCOCCUS NEOFORMANS) REPRESENT A SERIOUS HEALTH THREAT, ESPECIALLY TOIMMUNOCOMPROMISED PATIENTS AMONG WHOM INVASIVE CANDIDIASIS HAS HIGH MORBIDITY AND MORTALITY. CURRENTLY, IDENTIFICATIONOF FUNGAL PATHOGENS REQUIRES ISOLATION OF PURE CULTURES, FOLLOWED BY TESTING FOR SUBSTRATE ASSIMILATION AND FERMENTATION PATTERNS. THE LONG-TERM OBJECTIVE IS DEVELOPMENT OF A SERIES OF INNOVATIVE DIAGNOSTIC TESTS FOR SPECIFIC HUMAN FUNGAL PATHOGENS IN CLINICAL SAMPLES USING RAPID NUCLEIC ACID HYBRIDIZATION METHODOLOGY. THE IMMEDIATEGOAL IS TO FOCUS ON DEVELOPMENT OF A DIAGNOSTIC TEST FOR CANDIDA ALBICANS BASED ON HYBRIDIZATION OF A SPECIFIC DNA PROBE WITH HIGHLY AMPLIFIED RIBOSOMAL RNA (RRNA) SEQUENCES IN DISRUPTED YEAST CELLS. IN PHASE I, CANDIDA ALBICANS RRNAGENE FRAGMENTS WILL BE CLONED USING PURIFIED, IN VITRO RADIOLABELED 18S RRNA AS A PROBE. SUBCLONES FOUND TO MAP INA PUTATIVE UNIQUE REGION CORRESPONDING TO NUCLEOTIDES 642 TO702 OF PREVIOUSLY DESCRIBED EUKARYOTIC 18S RRNAS WILL BE DNASEQUENCED. DNA PROBES HOMOLOGOUS TO THIS AND OTHER REGIONS WILL BE ISOLATED OR SYNTHESIZED. THEY WILL THEN BE TESTED FOR SPECIFIC HYBRIDIZATION TO CANDIDA ALBICANS RNA IN PURE AND MIXED CULTURES. IN PHASE II, TEST FORMATS AND PROCEDURES WILL BE OPTIMIZED FOR USE WITH CLINICAL SAMPLES.

* information listed above is at the time of submission.

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