PREIMPLANTATION IDENTIFICATION OF TRANSGENIC EMBRYOS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1990
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
13753
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Integrated Genetics Inc
1 Mountain Rd, Framingham, MA, 01701
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Glenn M Monastersky
(508) 872-8400
Business Contact:
() -
Research Institution:
n/a
Abstract
THE OVERALL GOAL OF THIS STUDY IS TO EVALUATE THE USE OF EMBRYONIC BIOPSY AND POLYMERASE CHAIN REACTION (PCR) AMPLIFICATION TO IDENTIFY MICROINJECTED EMBRYOS IN WHICH THE FOREIGN DNA HAS BEEN INTEGRATED INTO THE GENOME. MICROINJECTED PRONUCLEAR EMBRYOS WILL BE CULTURED TO THE EIGHT-CELL STAGE, AND A SMALL SAMPLE OF EMBRYONIC CELLS WILLBE REMOVED WITH A BIOPSY PIPET AND LYSED TO PROVIDE A SMALL SAMPLE OF DNA FOR ANALYSIS. THIS METHOD OF GENOMIC ANALYSIS WILL MAINTAIN EMBRYO VIABILITY AND IS INTENDED TO RESTRICT EMBRYO TRANSFER TO THESMALL SUBSET OF MICROINJECTED EMBRYOS BEARING INTEGRATED TRANSGENES. PCR RESULTS OBTAINED FROM LYSATES OF THE BIOPSYSAMPLES WILL BE VALIDATED BY SPECIFIC RESTRICTION ENZYME DIGESTS DESIGNED TO DISCRIMINATE BETWEEN INTEGRATED AND NONINTEGRATED TRANSGENES AND BY ANALYSIS OF OFFSPRING. THESE TECHNIQUES WILL BE DEVELOPED AND EVALUATED IN PHASE I USING A MOUSE MODEL SYSTEM. THE METHODS RESULTING FROM THE PHASE I STUDY WILL BE UTILIZED IN PHASE II OF THE PROJECT, IN WHICH A HERD OF TRANSGENIC DAIRY GOATS, EXPRESSING MAMMARY-SPECIFIC TRANSGENES, WILL BE DEVELOPED FOR USE AS PHARMACEUTICAL BIOREACTORS.

* information listed above is at the time of submission.

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