TARGETING OF SELECTABLE MARKERS TO ANY GENETIC LOCUS

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 13978
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1990
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
1 Mountain Rd, Framingham, MA, 01701
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Gregory M Landes
 (508) 872-8400
Business Contact
Phone: () -
Research Institution
N/A
Abstract
IT IS THE LONG-TERM GOAL OF THIS RESEARCH TO DEVELOP A METHOD OF ORDERLY SEGMENTING THE HUMAN GENOME INTO 1-50 MB FRAGMENTS. THIS OBJECTIVE CAN BE ACHIEVED BY STREAMLINING THE SCREENING PROCESS OF HYBRID CLONES GENERATED BY CHROMOSOME FRAGMENT GENE TRANSFER. THE MOST EXPEDITIOUS SCREENING PROCESS IS ONE THAT INCLUDES A DOMINANT SELECTABLE MARKER NEAR THE LOCUS OF INTEREST. HOMOLOGOUS RECOMBINATION WILL BE USED TO DIRECT A DOMINANT SELECTABLE MARKER TO A SPECIFIC CHROMOSOMAL LOCATION ALLOWING THE TARGETED LOCUS TO BECOME PHENOTYPICALLY SELECTABLE. CELLS CONTAINING A CORRECTLY TARGETED DOMINANT MARKER ARE AN IDEALDONOR SOURCE FOR GOSS-HARRIS HYBRIDS. THE TRANSGENOMES PRODUCED BY THIS APPROACH WILL BE VALUABLE REAGENTS FOR MOLECULAR AND MEDICAL GENETIC RESEARCH. THEY WILL ALLOW FOR A RAPID MEANS OF SATURATING A LOCUS WITH GENETIC MARKERS. IN ADDITION, THE RESULTING ENRICHMENT OF ATARGETED GENETIC DISEASE LOCUS WILL FACILITATE THE IDENTIFICATION OF THE RESPONSIBLE DEFECT. AT THE GENOME LEVEL, A SET OF TARGETING VECTORS, EACH DIRECTED TO CHROMOSOMAL LOCATIONS SEPARATED BY 10-20 MB, COULD GENERATE AN ORDERED SET OF TRANSGENOMES. THESE REAGENTS WOULD ENHANCE CURRENT AND FUTURE HUMAN GENOME MAPPING EFFORTS.

* Information listed above is at the time of submission. *

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