Biochemical Detection of DNA Damage in Aquatic Species

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1 R43 ES07310-01,
Agency Tracking Number: 29417
Amount: $98,303.00
Phase: Phase I
Program: SBIR
Awards Year: 1995
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
800-12 Capitola Drive/Alston T, Durham, NC, 27713
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Raymond Tice
 () -
Business Contact
Phone: () -
Research Institution
For the biomonitoring of DNA damage in aquatic species exposed to genotoxic agents, an optimal methbe capable of detecting many classes of DNA damage in a variety of cell types, should provide data aindividual cell, should be adaptable to field studies, and should be sensitive, rapid, and cost effeelectrophoresis assay has been developed which appears to fulfill these criteria. The Single Cell Geindividual cells, the presence of DNA single strand breaks and alkali-labile sites. Other advantages(1) data that are obtained on the intercellular distribution of DNA damage; (2) extremely small numb(i.e., a few thousand cells); and (3) its speed, sensitivity, and simplicity. The research will charthis technique as an environmental biomarker for DNA damage using in vivo studies involving freshwatinvertebrates. In Phase II, research will be expanded to include on-site collection of species fromareas. When fully characterized, this technique will allow the monitoring of DNA damage in aquatic ptissue samples obtained either in the field or the laboratory.

* Information listed above is at the time of submission. *

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government