METHODS ARE NOW AVAILABLE FOR IDENTIFYING AND PURIFYING A VAST ARRAY OF ANTIGENS EXPRESSED ON TUMOR CELLS.

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 3067
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1985
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Intek Diagnostics, Inc. Forme
1450 Rollins Road, Burlingame, CA, 94010
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 PATRICIA A. NELSON
 PRINCIPAL INVESTIGATOR
 (415) 340-0530
Business Contact
Phone: () -
Research Institution
N/A
Abstract
METHODS ARE NOW AVAILABLE FOR IDENTIFYING AND PURIFYING A VAST ARRAY OF ANTIGENS EXPRESSED ON TUMOR CELLS. THE PURPOSE OF THE PROPOSED STUDIES IS TO DEVELOP METHODS FOR SENSITIZING T CELLS TO ANTIGENS THAT CAN BE PURIFIED FROM TUMOR CELL MEMBRANES, RATHER THAN SENSITIZING WITH INTACT TUMOR CELLS EXPRESSING THE ANTIGEN. PHASE I STUDIES WILL UTILIZE A MURINE TUMOR EXPRESSING A WELL-DEFINED TUMOR ASSOCIATED ANTIGEN (TAA), WHICH CAN BE READILY PURIFIED AND IS KNOWN TO ELICIT A MEASURABLE T-CELL RESPONSE. THESE STUDIES SHOULD PROVIDE INSIGHT INTO HOW TO HANDLE TAA SO AS TO RETAIN IMMUNOGENICITY AND TO PERMIT GENERATION OF BOTH CYTOTOXIC EFFECTOR T CELLS AND HELPER/DTH EFFECTOR T CELLS REACTIVE WITH THE TUMOR. STUDIES PERFORMED WITH BIOCHEMICALLY PROCESSED TAA AND BIOSYNTHETIC PLANAR LIPID MEMBRANES AS AN ANTIGEN-PRESENTING SURFACE SHOULD HELP DEVELOP METHODOLOGY FOR BYPASSING THE REQUIREMENT FOR BOTH INTACT TUMOR CELLS AND ANTIGEN-PRESENTING CELLS FROM THE PATIENT FOR IN VITRO SENSITIZATION OF T CELLS. MOREOVER, THIS TECHNOLOGY MAY PERMIT DEVELOPMENT OF HIGHLY IMMUNOGENIC SENSITIZING SURFACES, SINCE THE ARTIFICIAL MEMBRANES CAN BE MADE TO EXPRESS MUCH HIGHER DENSITY TAA THAN THE INTACT TUMOR CELL. PHASE II STUDIES WILL USE THESE METHODS TO DETERMINE WHETHER HUMAN TAA PURIFIED FROM SQUAMOUS CARCINOMA CELLS, USING MONOCLONAL ANTIBODIES THAT HAVE BEEN GENERATED AND CHARACTERIZED AT INTEK DIAGNOSTICS, CAN BE USED TO STIMULATE REACTIVE T CELLS FROM PATIENTS.

* information listed above is at the time of submission.

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