OF RNA

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1986
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
4712
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
International Biotechnologies
Box 1565, 275 Winchester Avenue, New Haven, CT, 06511
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
RODERIC M K DALE
PRINCIPAL INVESTIGATOR
(203) 562-3878
Business Contact:
() -
Research Institution:
n/a
Abstract
INTERNATIONAL TECHNOLOGIES HAS DEVISED A STRATEGY TO FACILITATE SEQUENCING METHODOLOGY BY USING PHAGE RNA POLYMERASES AND 3'-DEOXYNUCLEOTIDES AND MULTIPURPOSE VECTORS. THIS APPROACH IS EASIER AND FASTER THAN SANGER DIDEOXY SEQUENCING FOR SMALL FRAGMENTS, AS A FRAGMENT ONLY NEEDS TO BE CLONED IN ONE ORIENTATION TO SEQUENCE BOTH STRANDS. PROBLEMS ASSOCIATED WITH SECONDARY STRUCTURE IN SEQUENCING SHOULD BE ELIMINATED WITH THIS TECHNIQUE BECAUSE IT USES DOUBLE-STRANDED DNA AS TEMPLATE. THROUGH THE USE OFMULTIPURPOSE PIBI VECTORS, ONE COULD GO BACK AND FORTH BETWEEN SANGER DIDEOXY AND RNA POLYMERASE SEQUENCING DEPENDING UPON WHICH PROCEDURE GAVE OPTIMAL RESULTS.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government