Mass Spectrometric Immunoassay of Apolipoproteins

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R44HL072671-01
Agency Tracking Number: HL072671
Amount: $111,948.00
Phase: Phase I
Program: SBIR
Awards Year: 2003
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 (408) 804-1778
Business Contact
Phone: (480) 804-1778
Research Institution
DESCRIPTION (provided by applicant): Proposed in this Fast Track Phase I/Phase II proposal is the development of mass spectrometric immunoassays (MSIA) targeting apolipoproteins in human blood. Assays, utilizing antibody-derivatized affinity pipettes and MALDI-TOF mass spectrometry, will be developed for Apo-A, -C, -D, -E, -H and -J. These assays will have general use in studies investigating the quantitative modulation of, and point mutations and posttranslational modifications present in, the apolipoproteins. Phase I research will address the Specific Aim of optimizing affinity pipette chemistries for apolipoproteins. By reaching the Phase I milestone of high reproducibility, low non-specific binding qualitative assays (for the apolipoproteins when analyzed from small volumes (< 50 uL) of human blood), we will proceed into Phase II research. Phase II objectives include: 1) The use of enzymatically-active MALDI-TOF MS targets in conjunction with MSIA for structural characterization of the apolipoproteins, 2) The incorporation of quantitative methodologies into the assays, and 3) The development of a MSIA assay for common ApoE phenotypes. The final objective of the program is the development of kits and low-cost analytical platforms for the high-throughput analysis of apolipoproteins. Upon supply to the public, the approach will find initial applications in proteomic investigations linking protein variants with disease and potential long-term applications as phenotyping assay for use in clinical/diagnostic or drug administration applications.

* Information listed above is at the time of submission. *

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government