Mass Spectrometric Immunoassay of Apolipoproteins

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 4R44HL072671-02
Agency Tracking Number: HL072671
Amount: $1,029,846.00
Phase: Phase II
Program: SBIR
Awards Year: 2003
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
INTRINSIC BIOPROBES, INC.
INTRINSIC BIOPROBES, INC., 625 S SMITH RD, STE 22, TEMPE, AZ, 85281
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 RANDALL NELSON
 (408) 804-1778
 RNELSON@INTRINSICBIO.COM
Business Contact
 KEMMONS TUBBS
Phone: (480) 804-1778
Email: KTUBBS@INTRINSICBIO.COM
Research Institution
N/A
Abstract
DESCRIPTION (provided by applicant): Proposed in this Fast Track Phase I/Phase II proposal is the development of mass spectrometric immunoassays (MSIA) targeting apolipoproteins in human blood. Assays, utilizing antibody-derivatized affinity pipettes and MALDI-TOF mass spectrometry, will be developed for Apo-A, -C, -D, -E, -H and -J. These assays will have general use in studies investigating the quantitative modulation of, and point mutations and posttranslational modifications present in, the apolipoproteins. Phase I research will address the Specific Aim of optimizing affinity pipette chemistries for apolipoproteins. By reaching the Phase I milestone of high reproducibility, low non-specific binding qualitative assays (for the apolipoproteins when analyzed from small volumes (< 50 uL) of human blood), we will proceed into Phase II research. Phase II objectives include: 1) The use of enzymatically-active MALDI-TOF MS targets in conjunction with MSIA for structural characterization of the apolipoproteins, 2) The incorporation of quantitative methodologies into the assays, and 3) The development of a MSIA assay for common ApoE phenotypes. The final objective of the program is the development of kits and low-cost analytical platforms for the high-throughput analysis of apolipoproteins. Upon supply to the public, the approach will find initial applications in proteomic investigations linking protein variants with disease and potential long-term applications as phenotyping assay for use in clinical/diagnostic or drug administration applications.

* information listed above is at the time of submission.

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