Validation of Alcohol Exposure Biomarkers via Affinity Mass Spectrometry
Small Business Information
INTRINSIC BIOPROBES, INC., 2155 E. Conference Dr. Suite 104, TEMPE, AZ, 85284
AbstractDESCRIPTION (provided by applicant): The objective of this proposal is to validate potential biomarkers of excessive alcohol exposure discovered in the Phase 1 portion of this work (1R43AA016233-01). This will entail the development of pipettor tip based i mmuno-affinity mass spectrometry (AMS) devices for the routine and repetitive high throughput analysis of the biomarkers in question. Resultant devices will allow for both quantitative and qualitative differentiation of the markers of interest. With the su ccessful development of immuno-AMS devices for the biomarkers in question, they will be used to screen clinical samples collected by our clinical collaborators (Aurora Psychiatric Hospital, Wauwatosa, WI) in order to validate their performance. Final metri cs for the evaluation of the biomarker assays developed will be a clinical sensitivity and specificity of 85%, with coefficients of variation of lt15%. PUBLIC HEALTH RELEVANCE: Alcohol abuse has a significant impact on our society, yet there are no accurat e biomarker tests for prognostic, diagnostic or treatment efficacy assessment currently available. This application is to validate new biomarkers of excessive alcohol exposure using a novel in pipettor tip immuno-affinity mass spectrometry based assay. Cle arly, accurate and specific biomolecular assays are of great importance, positively impacting the associated health, financial and emotional repercussions of alcohol abuse.
* information listed above is at the time of submission.