Photofragmentation MS of Ion Mobility Separated Peptides

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$99,459.00
Award Year:
2003
Program:
SBIR
Phase:
Phase I
Contract:
1R43RR017139-01A1
Award Id:
65515
Agency Tracking Number:
RR017139
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
IONWERKS, INC., 2472 BOLSOVER, STE 255, HOUSTON, TX, 77005
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
JSCHULTZ
(713) 522-9880
AL@IONWERKS.COM
Business Contact:
(713) 522-9880
Research Institute:
n/a
Abstract
DESCRIPTION (provided by applicant): This Small Business Innovation Research Phase I project proposal is focused on the development of a commercial instrument capable of rapid, high-throughput identification of peptides based on matrix-assisted laser desorption ionization, ion mobility spectrometry, photodissociation, and time-of-flight mass spectrometry technology. The implications of the proposed instrument are vast but the most significant contribution is a time reduction in the identification of peptides from mixtures (e.g., protein digests). The keys to the project are the use of an ion mobility spectrometer as the separation device and a laser for ion dissociation. For example, an ion mobility spectrometer is capable of separating peptide mixtures with a resolution of 50-100 on the millisecond time-scale. This is a significant saving in time compared to the tens to hundreds of minutes needed by HPLC. The incorporation of a laser to induce fragmentation provides mass and chemical selectivity not afforded by other fragmentation means such as collision-induced dissociation or surface induced dissociation. Use of a laser also eliminates the need for numerous ion optical devices for fragmentation and focusing of ions, thereby simplifying the overall instrument design. The instrument can also be extended to other areas of chemical research simply by adjusting the laser wavelength.

* information listed above is at the time of submission.

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