Infrared Laser Desorption Ion Mobility Mass Spectrometer

Award Information
Agency:
Department of Health and Human Services
Branch:
N/A
Amount:
$199,418.00
Award Year:
2007
Program:
SBIR
Phase:
Phase II
Contract:
2R44RR020238-02A2
Agency Tracking Number:
RR020238
Solicitation Year:
2007
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
IONWERKS, INC.
3401 Louisiana St., Suite 355, HOUSTON, TX, 77002
Hubzone Owned:
N
Socially and Economically Disadvantaged:
Y
Woman Owned:
Y
Duns:
154074553
Principal Investigator
 MICHAEL UGAROV
 (713) 522-9880
 MVU@IONWERKS.COM
Business Contact
 MARIA AMBRIX
Phone: (713) 522-9880
Email: dempsey@ionwerks.com
Research Institution
N/A
Abstract
DESCRIPTION (provided by applicant): The development of an infrared laser desorption ionization ion mobility mass spectrometer (IR LDI IM MS) will be continued. The analysis of test biomolecules such as peptides and proteins in the Phase I of the project has demonstrated that the new system's performance is not only comparable, but, in some aspects, superior to the UV MALDI mode due to both mobility pre-separation of ion and softer nature of the IR MALDI. These results could provide a breakthrough in both top down proteomics as well as protein biomarker identification. Matrix-free laser desorption as well as efficient two-dimensional analysis of rat brain tissues and bacteria was also demonstrated. Based on the proof of capabilities of the technique in the Phase I, the instrument will be applied to the analysis of biological tissues. Both matrix-assisted and matrix-free approach will be used. The instrument will be upgraded in order to further increase the throughput, sensitivity and resolution. As part of this research, Ionwerks is collaborating with the mass spectrometry research group of Prof. Kermit Murray at Louisiana State University who are the experts in the use of IR lasers for the direct analysis of biomolecules. The utility of the IR LDI IM MS system spatial imaging of drugs of abuse, metabolite, lipids, and neuropeptides on and within rat brain tissue sections and the model Aplysia californica will be demonstrated. An advanced technique of the analysis of complex biological systems will be developed, which will have significant advantages compared to the conventional UV MALDI. Important commercial applications of this technology are in biomedical research and clinical diagnostics.

* information listed above is at the time of submission.

Agency Micro-sites

US Flag An Official Website of the United States Government