A NEW TUMOR-ASSOCIATED ANTIGEN, CALLED TENNESSEE ANTIGEN (TAG), HAS BEEN ISOLATED FROM 95% OF 154 PRIMARY HUMAN GASTRAINTESTINAL TUMORS.

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$44,150.00
Award Year:
1984
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
1405
Agency Tracking Number:
1405
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
P.o. Box 6275, Knoxville, TN, 37914
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
JOHN D. JORDAN
CO-DIRECTOR
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
A NEW TUMOR-ASSOCIATED ANTIGEN, CALLED TENNESSEE ANTIGEN (TAG), HAS BEEN ISOLATED FROM 95% OF 154 PRIMARY HUMAN GASTRAINTESTINAL TUMORS. IN 208 PATIENTS WITH COLORECTAL CARCINOMA, 81% SHOWED ELEVATED LEVELS OF TAG IN THEIR SERUM. IN THIS PROPOSAL, TAG WILL BE ISOLATED FROM EXTRACT OF ADENOCARCINOMA OF HUMAN GASTROINTESTINAL TRACT. MONOCLONAL ANTIBODIES (MOAB) AGAINST TAG WILL BE PRODUCED. SPLEEN CELLS FROM BALBIC MICE IMMUNIZED WITH TAG WILL BE USED TO FUSE WITH SP 2/0-AG 14 CELLS BY MEANS OF 30% POLYETHYLENE GLYCAL TO PRODUCE HYBRIDAMAS. CULTURE FLUIDS FROM HYBRIDOMAS WILL BE SCREENED FOR ANTIBODIES BY ENZYME IMMUNOASSAY. AFTER IDENTIFYING THE CULTURES PRODUCING SPECIFIC ANTIBODIES, THE HYBRIDOMA CELLS WILL BE GROWN AND CLONED BY LIMITING DILUTION IN FLUID PHASE. LINES PRODUCING MOAB WILL BE INJECTED INTO BALBIC MICE TO PRODUCE MOAB-CONTAINING ASCITES FLUID. THE MOAB WILL FACILITATE ANALYSIS OF STRUCTURE AND GENETIC ORIGIN OF TAG. IT CAN BE USED AS PART OF A PANEL OF MOAB TO IMMUNOLOGICALLY TYPE THE ANTIGENIC EXPRESSION OF COLORECTAL CARCINOMAS. COMMERCIALLY, MOAB CAN BE USED FOR DEVELOPING A SENSITIVE RADIOIMMUNOSSAY FOR DIAGNOSIS OF COLORECTAL CARCINOMAS. IT MAY BE USEFUL IN THE TREATMENT OF HUMAN COLORECTAL CARCINOMAS.

* information listed above is at the time of submission.

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