Chloroplast Genetic Engineering to Produce Diagnostic Antigens and Vaccines

Award Information
Agency: Department of Defense
Branch: Army
Contract: W81XWH-06-C-0009
Agency Tracking Number: A052-175-1017
Amount: $119,911.00
Phase: Phase I
Program: SBIR
Awards Year: 2005
Solicitation Year: 2005
Solicitation Topic Code: A05-175
Solicitation Number: 2005.2
Small Business Information
KUEHNLE AGROSYSTEMS CO. LLC
2800 Woodlawn Drive, Suite 281, Honolulu, HI, 96822
DUNS: 142296982
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Michele Champagne
 Senior Researcher
 (808) 753-2693
 KASLLC@hawaii.rr.com
Business Contact
 Gordon Wallace
Title: President
Phone: (808) 753-3170
Email: KASLLC@hawaii.rr.com
Research Institution
N/A
Abstract
Plant chloroplasts are capable of producing significant quantities of high quality bioengineered proteins with biomedical applications. Improving the output of therapeutic antibodies, vaccines and diagnostic antigens through use of our pliable expression platform will provide an important technological advance compared with the current mammalian and microbial cell culture platforms. Our innovative approach combines a next-generation plant platform, for rapid line production, with established downstream processing and a novel genetic technology that permits site-directed insertion of one or more transgenes in chloroplasts. This platform advance has the potential for significant production of antigens, such as for the hantavirus, in contained, biosecure facilities under conditions of Good Manufacturing Practice. We propose in this Phase I project to develop the technical feasibility for rapid production of hantaviral gene products using our technology for chloroplast transgenesis in our target platform. Production of hantavirual proteins using the enabling strategies in Phase I is key to our Phase II goal of applying the technology collaboratively to produce sufficient quality antigens for diagnostic and immunogenicity assays.

* information listed above is at the time of submission.

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