AN IMAGE ACQUISITION AND PROCESSING SYSTEM FOR THE ANALYSIS OF FLUORESCENCE FROM STAINED DNA GELS

Award Information
Agency:
Department of Energy
Branch
n/a
Amount:
$486,000.00
Award Year:
1988
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
7017
Agency Tracking Number:
7017
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Po Box 1567, 3853 Research Park Dr, Ann Arbor, MI, 48106
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Mr Ron Mckean
(313) 769-8500
Business Contact:
() -
Research Institute:
n/a
Abstract
EVALUATION OF DAMAGED DNA COMPONENTS IS NORMALLY PERFORMED VIA AGAROSE GEL ELECTROPHORESIS OF DNA SAMPLES STAINED WITH FLUORESCING DYE. ALTHOUGH THIS METHOD PROVIDES ADEQUATE SENSITIVITY AND RESOLUTION IN THE IDENTIFICATION OF DNA COMPONENTS, THE LACK OF AVAILABLE INSTRUMENTATION TO CONVERT THE FLUORESCING IMAGE INTO A DIGITIZED RECORD FOR COMPUTER ENTRY AND THE LACK OF ANY TECHNIQUE FOR STANDARDIZING ANALYSES PERFORMED UNDER VARYING CONDITIONS SEVERELY LIMIT THE USEFULNESS OF THIS ANALYSIS. THE GOAL OF THIS PROJECT IS TO DEVELOP AN INSTRUMENT THAT MAKES IT POSSIBLE TO DIGITIZE STAINED DNA GELS DIRECTLY INTO ANY COMPUTER (VIA A STANDARD COMMUNICATIONS INTERFACE), STANDARDIZE THE RESULTS, AND PERFORM STATISTICAL ANALYSIS OF THE DATA. THIS INSTRUMENT WILL QUICKLY SCAN THE GEL USING AN OPTICAL SYSTEM THAT HAS HIGH PHOTOMETRIC RESOLUTION AND VERY HIGH SPATIAL PRECISION. THE DIGITIZED DATA WILL THEN BE ELECTRONICALLY PREPROCESSED (BACKGROUND SUBTRACTION, FILTERING, DATA COMPRESSION, ETC.) TO PROVIDE CORRECT, NOISE-FREE DATA STRUCTURED FOR STORAGE WITH MINIMAL MEMORY REQUIREMENTS. THE STORED DATA WILL THEN BE AVAILABLE FOR USE IN IMAGING THE GEL, PRODUCING STATISTICAL COMPARISONS, AND GENERATING GRAPHIC DISPLAYS. THE DATA WILL BE ARCHIVABLE USING MEDIA SUCH AS MAGNETIC TAPES OR DISKS. THIS INSTRUMENT WILL OFFER A SOLUTION TO PROBLEMS LONG ASSOCIATED WITH AGAROSE GEL ANALYSIS OF DNA. THE DIRECT QUANTIFICATION OF FLUORESCENCE IN STAINED DNA GELS WILL ALLOW IMMEDIATE ACCESS TO THE DATA BY A COMPUTER, INSTANTLY PROVIDING SUCH INFORMATION AS AVERAGE MOLECULAR WEIGHT. THE INSTRUMENT WILL ENABLE DIRECT COMPARISON OF SEPARATE ANALYSES PERFORMED UNDER VARYING CONDITIONS, ALLOWING FOR COMPLETE NORMALIZATION OF RESULTS AND GENERATION OF STATISTICAL DATA INVOLVING MANY INDIVIDUAL TESTS.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government