CENTRIFUGAL FAST CHROMATOGRAPHY

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1988
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
8043
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Large Scale Biology Corp
7503 Standish Pl, Rockville, MD, 20855
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
OUR OBJECTIVE IS TO REVOLUTIONIZE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY USING NEW PRINCIPLES AND A NEW ANALYTICAL SYSTEM WHICH ALLOWS MULTIPLE-PARALLEL ANALYSIS OFMANY SAMPLES WITH HIGH RESOLUTION, AUTOMATIC DATA ACQUISITION, AND DATA ANALYSIS AND DISPLAY. THE HIGH PRESSURES REQUIRED FOR HIGH RESOLUTION ARE OBTAINED BY CENTRIFUGAL FORCE, WHICH ALSO MOVES SAMPLES FROM A TRANSFER DISC TO THE CHROMATOGRAPHIC COLUMNS. A GRADIENT SEGMENTATION DEVICE CUTS ONE ELUTION STREAM INTO SMALL FRACTIONS WHICH ARE INTRODUCED EQUALLY INTO ALL COLUMNS. AN ELUTING DENSITY GRADIENT, WHEN COMBINED WITH CENTRIFUGAL FORCE, MAINTAINS THE SAMPLE ZONE, AND ALL SEPARATED ZONES INPOSITION, AND PERPENDICULAR TO A RADIUS THROUGH THE COLUMN. THE DENSITY GRADIENT ALSO KEEPS TIGHT ZONES AS THEY MOVE THROUGH THE FLOW CELL. TWO STREAM SEGMENTATION DEVICES ARE PROVIDED TO ALLOW, IN CONCERT WITH SUITABLE VALVING, FLOW IN EITHER INBOARD OR OUTBOARD DIRECTION THROUGH THE COLUMN. OUTBOARD ELUTION WITH THE FLOW CELL CENTRIPETAL TO THE COLUMN IS USED WITH GRADIENTS OF DECREASING DENSITY, WHILE ELUTION IN AN INBOARD DIRECTION IS USED WITH GRADIENTS OF INCREASING DENSITY. SINCE THE SYSTEM HAS NO PUMPS, RUNS MANY COLUMNS IN PARALLEL, AND USES ONE OPTICAL SYSTEM AND MICROPROCESSOR FOR DATA ACQUISITION AND ANALYSIS,AND PROVIDES ANALYSES AT A HIGH RATE, WE BELIEVE THAT THIS SYSTEM WILL, WHEN PERFECTED, SUPPLANT A LARGE FRACTION OF THE HPLC SYSTEMS IN PRESENT USE.

* information listed above is at the time of submission.

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