DNA polymerase for single molecule DNA sequencing

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$422,170.00
Award Year:
2001
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Agency Tracking Number:
2R44HG002292-02
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
LI-COR, INC.
BOX 4425, 4421 SUPERIOR ST, LINCOLN, NE, 68504
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
JOHN WILLIAMS
() -
Business Contact:
(402) 467-3576
LYLEM@LICOR.COM
Research Institution:
n/a
Abstract
DESCRIPTION (provided by applicant): We are developing a new technology for single molecule DNA sequencing based on the incorporation of dNTPs labeled on the g-phosphate with fluorescent dyes. The label is cleaved from the nucleotide along with pyrophosphate when the nucleotide is incorporated into DNA by a polymerase. The released label is sorted from intact g-dNTPs in a microfluidics system and is identified in real time by single molecule fluorescence detection optics. In this proposal, we develop a novel nucleotide chemistry to enable the sorting scheme and we use directed evolution methods to adapt polymerases for utilization of the g-dNTPs. Single molecule sequencing promises to reduce the cost of DNA sequencing by several orders of magnitude compared to current methods. PROPOSED COMMERCIAL APPLICATIONS: A genomic DNA sequencing technology that reduces cost and eliminates the need for cloning, physical mapping and electrophoresis would have great appeal to both the academic and industrial genomic research communities.

* information listed above is at the time of submission.

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