Electrokinetic Method For Single Molecule DNA Sequencing

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43HG003610-01
Agency Tracking Number: HG003610
Amount: $99,950.00
Phase: Phase I
Program: SBIR
Awards Year: 2005
Solicitation Year: 2005
Solicitation Topic Code: N/A
Solicitation Number: PHS2005-2
Small Business Information
Li-Cor, Inc., 4308 Progressive Ave, Lincoln, NE, 68504
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 JOHN WILLIAMS
 (402) 467-0732
 jwilliams@licor.com
Business Contact
Phone: (402) 467-0700
Research Institution
N/A
Abstract
DESCRIPTION (provided by applicant): We propose a new approach to single-molecule DMA sequencing that can be done in a simple microtiter plate fitted with two electrodes. Over two-hundred different single DMA molecules would be sequenced simultaneously in a single well (maximum 10 nt/s per molecule) with read lengths 20 kb or more. Sequencing cost is potentially lower with this method compared to other long read approaches because of a high degree of multiplexing and because a simple microtiter plate substitutes for expensive micro or nanofabricated devices. While novel in concept, the proposed "electrokinetic" sequencing method would utilize modified polymerases and phosphate-labeled nucleotides from ongoing research on an alternative "charge-switch" method of single molecule sequencing. Ultrabright fluorescent nanoparticles and a prototype two-electrode sequencing chamber will be developed in Phase I. Particle transport kinetics will be characterized using the prototype chamber.

* Information listed above is at the time of submission. *

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