Electrokinetic Method For Single Molecule DNA Sequencing

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$99,950.00
Award Year:
2005
Program:
SBIR
Phase:
Phase I
Contract:
1R43HG003610-01
Agency Tracking Number:
HG003610
Solicitation Year:
2005
Solicitation Topic Code:
n/a
Solicitation Number:
PHS2005-2
Small Business Information
LI-COR BIOSCIENCES, INC.
Li-Cor, Inc., 4308 Progressive Ave, Lincoln, NE, 68504
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
JOHN WILLIAMS
(402) 467-0732
jwilliams@licor.com
Business Contact:
(402) 467-0700
Research Institution:
n/a
Abstract
DESCRIPTION (provided by applicant): We propose a new approach to single-molecule DMA sequencing that can be done in a simple microtiter plate fitted with two electrodes. Over two-hundred different single DMA molecules would be sequenced simultaneously in a single well (maximum 10 nt/s per molecule) with read lengths 20 kb or more. Sequencing cost is potentially lower with this method compared to other long read approaches because of a high degree of multiplexing and because a simple microtiter plate substitutes for expensive micro or nanofabricated devices. While novel in concept, the proposed "electrokinetic" sequencing method would utilize modified polymerases and phosphate-labeled nucleotides from ongoing research on an alternative "charge-switch" method of single molecule sequencing. Ultrabright fluorescent nanoparticles and a prototype two-electrode sequencing chamber will be developed in Phase I. Particle transport kinetics will be characterized using the prototype chamber.

* information listed above is at the time of submission.

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