SUMO Fusions to Enhance Expression of Membrane Proteins

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43GM074316-01
Agency Tracking Number: GM074316
Amount: $206,308.00
Phase: Phase I
Program: SBIR
Awards Year: 2005
Solicitation Year: 2005
Solicitation Topic Code: N/A
Solicitation Number: PHS2005-2
Small Business Information
Lifesensors, Inc., 271 Great Valley Pky, Malvern, PA, 19355
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 (610) 644-8845
Business Contact
Phone: (610) 644-8845
Research Institution
DESCRIPTION (provided by applicant): Membrane proteins have crucial roles in many cellular and physiological processes. Understanding the structures and functions of these proteins is academically and commercially important. However, the lack of efficient methods for expressing membrane proteins has created a bottleneck. While one third of all proteins are membrane proteins, less than 100 membrane protein structures have been solved to the date. Among membrane proteins, G protein coupled receptors (GPCR) represent a large family of about 1000 proteins in human cells and are the most important drug targets. We propose the development of the SUMO-Fusion system to enhance expression and purification of a wide variety of membrane proteins in E. coli. The key features of the SUMO Fusion technology are: 1) attachment of the C-terminus of SUMO protein to the N terminus of a membrane protein to act as a chaperone, enhancing the qualitative and quantitative expression of the membrane protein; 2) introduction of N-terminal 6xHis-SUMO to act as a tag to allow rapid purification; 3) exploitation of SUMO proteases to provide efficient cleavage of SUMO fusions, thereby generating membrane proteins with native N-termini. The goal of this proposal is to develop and improve the SUMO fusion system for enhancement of expression and purification of model membrane proteins in E. coli.

* Information listed above is at the time of submission. *

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