HYBRID CELL FORMATION BY TARGETED ELECTROFUSION

Award Information
Agency:
National Science Foundation
Branch
n/a
Amount:
$200,000.00
Award Year:
1988
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
2655
Agency Tracking Number:
2655
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Litron Laboratorytories Ltd (Currently LITRON LABORATORIES, LTD.)
1351 Mt Hope Ave #207, Rochester, NY, 14620
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Andrew M Tometsko Phd
Director Of Research
(716) 275-4008
Business Contact:
() -
Research Institution:
n/a
Abstract
CELL FUSION PROVIDES BIOTECHNOLOGISTS WITH THE ABILITY TO GENERATE UNIQUE CELL TYPES BY MIXING THE GENETIC MATERIAL OFTWO PARENT CELLS. THE GENERATION OF IMMORTAL ANTIBODY PRODUCTING HYBRIDOMAS BY FUSING ANTIBODY PRODUCING SPLEEN CELLS WITH MYELOMA CELLS PROVIDES AN EXAMPLE OF THE UTILITY OF CELL FUSION TECHNOLOGY. HOWEVER, THE WIDELY USED CHEMICAL FUSION PROCEDURES, BASED ON POLYETHYLENEGLYCOL (PEG) ARE LABOR INTENSIVE, TIME CONSUMING AND COSTLY, AND THEY PROVIDE RELATIVELY LOW YIELDS OF THE DESIRED HYBRIDOMA.THE APPLICATION OF ELECTROFUSION (RATHER THAN CHEMICAL FUSION) IN CONJUNCTION WITH METHODS FOR TARGETING THE CELL POPULATIONS PRIOR TO FUSION, SHOULD PROVIDE AN IMPORTANT ADVANCE IN HYBRID CELL TECHNOLOGY. AS A RESULT OF THIS RESEARCH PROGRAM, WE WILL BE ABLE TO OBTAIN HIGHER YIELDS OFHYBRID CELLS, IN A SHORTER TIME AND AT A LOWER COST THAN PRESENT FUSION METHODS. THE HYBRIDOMA MODEL SYSTEM WILL PROVIDE THE TECHNOLOGICAL BASIS FOR EVENTUALLY TARGETING OTHER TYPES OF PLANT AND ANIMAL CELLS FOR ELECTROFUSION.

* information listed above is at the time of submission.

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