GENETIC TOXICOLOGY TESTING BY HIGH-SPEED FLOW CYTOMETRY

Award Information
Agency:
Department of Health and Human Services
Branch:
N/A
Amount:
$293,000.00
Award Year:
1987
Program:
SBIR
Phase:
Phase II
Contract:
N/A
Agency Tracking Number:
4940
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Litron Laboratorytories Ltd
1351 Mount Hope Avenue, Suite 207, Rochester, NY, 14620
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
N/A
Principal Investigator
 Andrew M Tometsko
 Principal Investigator
 (716) 275-4008
Business Contact
Phone: () -
Research Institution
N/A
Abstract
GENOTOXIC CHEMICALS ARE AN EXISTING WIDESPREAD HEALTH HAZARDTO THE HUMAN POPULATION. ADVANCES IN GENETIC TOXICOLOGY TESTING HAVE MADE IT POSSIBLE TO ASSAY POTENTIAL MUTAGENS, CARCINOGENS, TERATOGENS, AND CLASTOGENS IN THE ENVIRONMENT. HOWEVER, MANY CURRENTLY AVAILABLE MAMMALIAN CELL TESTS ARE COSTLY, TIME CONSUMING, AND LABOR INTENSIVE. THIS RESEARCH PROGRAM IS DIRECTED TOWARD THE APPLICATION OF HIGH-SPEED FLOW CYTOMETRY (FCM) TO EXPEDITE GENETIC TOXICOLOGY TESTING USING MAMMALIAN CELL SYSTEMS. THE FLOW CYTOMETER IS ABLE TOANALYZE MORE THAN 100,000 CELL/MINUTE AND THUS SHOULD BE ABLE TO PROVIDE (1) FASTER ANALYSIS OF TARGET CELLS, (2) LARGER POPULATIONS OF CELLS FOR BETTER STATISTICS, (3) THE POTENTIAL FOR AUTOMATING MAMMALIAN CELL TESTS, AND (4) POTENTIAL FOR LOWERING THE OVERALL COST OF MAMMALIAN CELL TESTS. DURING PHASE I OF THIS PROGRAM, FCM WILL BE USED TO AUTOMATE THE CELL AND DATA PROCESSING ASPECTS OF THE AGENTS TO CAUSE CHROMOSOMAL ABERRATIONS IN TARGET CELLS. WITH THE CURRENTLY AVAILABLE METHOD, CELL COUNTS ARE OBTAINED ON A RELATIVELY SMALL PERCENTAGE OF THE TOTAL CELL POPULATION. FCM WILL MAKE IT POSSIBLE TO INCREASE THE NUMBER OF CELLS COUNTED IN THE MICRONUCLEUS ASSAY 200 TO 300 TIMES, COMPAREDTO THE MANUAL METHOD. PHASE II WILL APPLY FCM TO ADDITIONALMAMMALIAN CELL TESTING PROTOCOLS. FCM AUTOMATION HAS FAR- REACHING COMMERCIAL APPLICABILITY FOR TOXICOLOGICAL TESTING.

* information listed above is at the time of submission.

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