COLOR BASED AUTOMATED MICRONUCLEI ASSAY

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$500,000.00
Award Year:
1993
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
16578
Agency Tracking Number:
16578
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
1004 Littlestown Pike, Westminster, MD, 21157
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Loats, Harry L
Principal Investigator
(410) 876-8055
Business Contact:
1 R43 ES05872-01
() -
Research Institution:
n/a
Abstract
THIS PROJECT IS FOR THE DEVELOPMENT OF AN AUTOMATED COLOR BASED MICRONUCLEI ASSAY SYSTEM BASED ON RESULTS OF A PROTOTYPE MS-DOS COLOR ANALYSIS SYSTEM. THE SYSTEM WILL AUTOMATICALLY LOCATE AND COUNT POLYCHROMATIC ERYTHROCYTES (PCE'S), MICRONUCLEATED POLYCHROMATIC ERYTHROCYTES (MPCES) AND NORMOCHROMATIC ERYTHROCYTES (NCES). DURING PHASE I, A NUMBER OF CRITICAL VARIABLE PARAMETERS PREVIOUSLY DEVELOPED WILL BE ADAPTED INTO A USER-FRIENDLY INTERACTIVE PROGRAM CAPABLE OF RAPIDLY SELECTING THE APPROPRIATE PARAMETERS FOR EACH NEW SLIDE PREPARATION. THESE PARAMETERS INCLUDE: THE LEVEL OF BACKGROUND STRAIN; CRITERIA FOR ACCEPTABLE ERYTHROCYTE SIZE RANGE AND ROUNDNESS; AND COLOR DISCRIMINATION. COLOR DISCRIMINATION IS THE KEY ELEMENT FOR DIFFERENTIATION. THE SYSTEM WILL BE IMPLEMENTED ON A MACINTOSH IIFX COMPUTER. IT WILL INCLUDE A CAPABILITY TO AUTOMATICALLY PROCESS MULTIPLE SLIDES AND PRODUCE AUTOMATIC REPORTS INCLUDING APPROPRIATE STATISTICAL ANALYSIS. BY CAPTURING ALL IMAGES WITH SCORED MICRONUCLEI THE SYSTEM PROVIDES A NECESSARY AUDIT TRAIL FOR REGULATORY OVERVIEW.

* information listed above is at the time of submission.

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