CHEMILUMINESCENCE IMMUNOASSAY FOR INSULIN

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1987
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
7229
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
London Diagnostics Inc
10300 Valley View Rd Suite 107, Eden Prairie, MN, 55344
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
RUSSELL C HART PHD
(612) 941-3812
Business Contact:
() -
Research Institution:
n/a
Abstract
THE AIM OF THIS PROJECT IS TO DEMONSTRATE THE FEASIBILITY OF AN IMMUNOASSAY FOR INSULIN, USING ACRIDINIUM ESTERS AS A CHEMILUMINESCENT LABEL. THIS WILL LEAD TO A TECHNIQUE FOR THE DETECTION OF POLYPEPTIDE HORMONES THAT IS FASTER AND MORE SENSITIVE, AND REMOVES THE NEED FOR (125)I-LABELED PEPTIDES OR ANTIBODIES. THE PROJECT WILL EXPLORE BOTH TRADITIONAL IMMUNOASSAYS IN WHICH THE INSULIN MOLECULE IS LABELED AND "SANDWICH"-TYPE ASSAYS IN WHICH INSULIN ANTIBODIES ARE USED. IN THE FIRST CASE, LABELED INSULIN WILL BE PURIFIED BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) AND DIRECTLY SUBSTITUTED AS THETRACER IN A RADIOIMMUNOASSAY (RIA) PROCEDURE. FOR THE IMMUNOMETRIC ASSAY, ANTIBODIES TO INSULIN WILL BE LABELED AND PURIFIED BY GEL FILTRATION CHROMATOGRAPHY. THE LABELED PEPTIDE AND ANTIBODY WILL BE COMPARED FOR STABILITY AGAINST A COMMERCIALLY AVAILABLE RIA USING (125)I-LABELED INSULIN. THE ASSAY WILL SERVE AS A MODEL FOROTHER PEPTIDE HORMONE ASSAYS THAT REQUIRE HIGH SENSITIVITY AND STABLE REAGENTS. THE PROJECT WILL INVOLVE LIMITED ASSAY DEVELOPMENT, COMPARISON WITH A COMMERCIAL RIA, AND A LIMITED TRIAL WITH PLASMA SAMPLES. THE MULTIMILLION-DOLLAR HORMONE RIA MARKET MIGHT INCREASE IFMORE STABLE, SAFER, CHEAPER, AND SIMPLER ASSAYS WERE AVAILABLE USING CHEMILUMINESCENCE DETECTION TECHNIQUES.

* information listed above is at the time of submission.

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