Single Cell Genomics of Hyperthermophiles

Award Information
Agency:
Department of Energy
Branch
n/a
Amount:
$100,000.00
Award Year:
2002
Program:
SBIR
Phase:
Phase I
Contract:
DE-FG02-02ER83484
Award Id:
57108
Agency Tracking Number:
70588S02-I
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Lucigen Corp. (Currently LUCIGEN CORPORATION)
2120 W. Greenview Drive, Middleton, WI, 53562
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
David Mead
(608) 831-9011
dmead@lucigen.com
Business Contact:
David Mead
70588
(608) 831-9011
dmead@lucigen.com
Research Institution:
n/a
Abstract
70588 Modern methods of molecular analysis reveal an astonishing discrepancy between the diversity and numbers of organisms present in natural habitats and the relatively few capable of being cultured. Single Cell Genomics is a new initiative to develop the tools and methods for extracting cultivation-resistant genetic diversity in a form that is useful for whole genome sequence analysis and expression screening. This project will apply these tools to discover valuable hyperthermophilic enzymes and sequence information from a class of microorganisms especially difficult to grow in the lab. In Phase I, single microorganisms will be isolated from a diverse mixture using microscopy and a micromanipulator. Specific tasks include the isolation of a single molecule of intact genomic DNA, unbiased amplification with high fidelity, ligation to a new dual orientation zero-background cloning vector, and transformation into a new E. coli strain. A random genomic library from a single microbial cell adequate for 10X sequence coverage will be constructed. The long-term goals of this research are to develop a phylogenetically diverse set of genomic libraries from single cells of culture-resistant hyperthermophiles and screen them for commercially valuable enzymes. Commercial Applications and Other Benefits as described by the awardee: The technology could result in sales of reagent kits, a single cell genomic DNA cloning service, and hyperthermophilic genomic libraries. Enzyme activity screens for hyperthermophilic laccase and xylanase activities should provide environmentally friendly new reagents for the fiber processing and wood pulping industries.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government