N/A

Award Information
Agency:
National Science Foundation
Branch:
N/A
Amount:
$99,052.00
Award Year:
2001
Program:
SBIR
Phase:
Phase I
Contract:
0109756
Agency Tracking Number:
0109756
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Lucigen Corporation (vice WI Corp)
2120 W Greenview Dr.,Suite 9, Middleton, WI, 53562
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
N/A
Principal Investigator
 Thomas Schoenfeld
 (608) 831-9011
 tschoenfeld@microgen.org
Business Contact
 David Mead
Title: President
Phone: (608) 831-9011
Email: dmead@microgen.org
Research Institution
N/A
Abstract
This Small Business Innovation Research (SBIR) Phase I project will develop new thermostable bacteriophage and archaeaviral DNA polymerases for use as improved reagents for DNA amplification, sequence analysis and single nucleotide polymorphism (SNP) detection. This will be accomplished by direct isolation of thermophilic bacteriophage and archaeaviral genomes from hot springs, construction of expression libraries and screening for novel thermostable DNA polymerase activities. The proposed approach is expected to be significantly more rapid and comprehensive than traditional approaches that have been used for enzyme discovery. These traditional approaches rely on the limiting intermediate step of culturing a microbe and its cognate virus. In contrast, the proposed approach on this project will allow screening of all expressible viral DNA polymerases present, including those from unculturable phage that is believed to predominate in the environment. The primary commercial application of this project will be in the marketing of novel thermostable DNA polymerases to organizations involved in genomics research for use as improved reagents for specific molecular biology methods

* information listed above is at the time of submission.

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