Portable System for Sample Preparation and Differentiation of Pathogens at Strain Level

Award Information
Agency: Department of Defense
Branch: Office for Chemical and Biological Defense
Contract: W911SR-04-P-0049
Agency Tracking Number: C041-107-0050
Amount: $100,000.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: CBD04-107
Solicitation Number: 2004.1
Solicitation Year: 2004
Award Year: 2004
Award Start Date (Proposal Award Date): 2004-04-21
Award End Date (Contract End Date): 2004-10-21
Small Business Information
7607 Eastmark Drive, Suite 102, College Station, TX, 77840
DUNS: 184758308
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Adrian Denvir
 Sr. Research Scientist
 (979) 693-0017
Business Contact
 G. Hitchens
Title: Vice President
Phone: (979) 693-0017
Email: duncan.hitchens@lynntech.com
Research Institution
During the anthrax attacks in 2001, investigators had difficulty in differentiating the strains apart because B. anthracis has a low level of genetic variability. This event demonstrated the need for rapid and precise molecular subtyping technologies. Lynntech proposes to develop an ultra-sensitive quantum dots-based molecular beacon fluorogenic reporter system to identify the presence of specific pathogen species at the strain level. The technology will be incorporated with the surface sampling device and the portable PCR-unit being developed by Lynntech. Molecular beacon (MB) is a hair-pin shaped oligonucleotide probe that has a fluorescent dye and a quencher located at each end of the strand. MBs become fluorescent upon hybridization and they have selectivity for single base-pair mismatch identification. MBs modified with fluorogenic quantum dots of characteristic emission colors will allow more sensitive multiplex detection of DNA sequences with much simpler and less expensive instrumentation. Lynntech will demonstrate the technology's ability to discriminate between different strains of E. Coli. Lynntech will also demonstrate the ability to differentiate different amplicons obtained from similar species such as Escherichia coli and Salmonella. These assays will be carried out entirely in sealed PCR tubes, enabling fast and direct detection of E. Coli. in an automated format.

* Information listed above is at the time of submission. *

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