TRANSFORMATION STUDIES WITH THIOBACILLUS FERROOXIDANS AS A PREREQUISITE FOR STRAIN IMPROVEMENT FOR STRATEGIC METALS RECOVERY

Award Information
Agency:
National Science Foundation
Branch
n/a
Amount:
$43,876.00
Award Year:
1989
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
10675
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Martek Corp
640 Dobbin Rd, Columbia, MD, 21045
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Jon C Cox
Principal Investigator
(301) 740-0081
Business Contact:
() -
Research Institution:
n/a
Abstract
ESTIMATES THAT SUGGEST THAT METALLIC ORE DEPOSITS ARE BEING DEPLETED THOUGH THE REQUIREMENT FOR METALS (ESPECIALLY STRATEGICALLY IMPORTANT ONES) ARE INCREASING. TOXIC-METAL RICH WASTE OCCURS IN SEVERAL INDUSTRIAL PROCESSES, AND ORGANISMS SUCH AS THIOBACILLUS FERROOXIDANS COULD BE USED TO DETOXIFY THIS WASTE. OUR ABILITY TO TAKE FULL ADVANTAGE OF THE VALUE OF MICROBIAL WASTE METAL MANAGEMENT IS HAMPEREDBY OUR LACK OF KNOWLEDGE ABOUT THE GENETICS AND BIOCHEMISTRYOF T.FERROOXIDANS. TO DATE, THERE ARE NO PUBLISHED PROCEDURES FOR THE GENETIC TRANSFORMATION OF THE ORGANISM. TRANSFORMATION WILL PERMIT SPECIFIC MODIFICATIONS TO BE MADETO T.FERROOXIDANS FOR THE PURPOSE OF IMPROVING ITS METAL PURIFYING CAPACITY. THE RESEARCHERS WILL USE CELLS GROWN ATPH5 AND PLASMIDS CONFERRING KANAMYCIN RESISTANCE TO PERFORM TRANSFORMATION OF THE ORGANISM (PH5 IS A COMPROMISE BETWEEN THE PH FOR GROWTH AT WHICH DNA IS UNSTABLE AND PH8, THE OPTIMUM FOR MOST TRANSFORMATIONS BUT WHERE T.FERROOXIDANS ISINACTIVE). THEY ALREADY POSSESS KANAMYCIN SENSITIVE AND RESISTANT STRAINS THAT WERE OBTAINED FROM CULTURES GROWN AT PH5 ON SULFUR-CONTAINING MEDIUM.

* information listed above is at the time of submission.

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