MODIFIED SURFACES FOR CHEMICALLY DEFINED CELL CULTURE

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 14057
Amount: $15,760.00
Phase: Phase I
Program: SBIR
Awards Year: 1990
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Mattek Corp
200 Homer Ave, Ashland, MA, 01721
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Mitchell Klausner
 (508) 881-6771
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE PRIMARY GOAL OF THIS RESEARCH IS THE DEVELOPMENT OF NOVEL PLASTICS ON WHICH TO CULTURE CELLS IN SERUM-FREE MEDIA. CURRENTLY, BIOLOGICAL ATTACHMENT AIDS MUST BE USED FOR ANCHORAGE-DEPENDENT CELLS TO ATTACH AND GROW NORMALLY INTHE ABSENCE OF SERUM. LOW-TEMPERATURE GAS PLASMA TECHNIQUES WILL BE EMPLOYED TO MODIFY THE SURFACES OF POLYSTYRENE CULTUREWARE. SUCCESS WILL BE DETERMINED BY THE ABILITY OF THE MODIFIED SURFACES TO FOSTER ATTACHMENT AND GROWTH OF ANCHORAGE-DEPENDENT CELLSIN CHEMICALLY DEFINED MEDIA. IN ADDITION, A SECONDARY GOAL WOULD BE ACHIEVED IF A SURFACE WAS DEVELOPED THAT ENHANCED CELL ATTACHMENT AND GROWTH IN VERY LOW SERUM CONCENTRATIONS. TWO REPRESENTATIVE CELL TYPES WILL BE USED: PRIMARY CHICK EMBRYO FIBROBLASTS AND CHINESE HAMSTER OVARY CELLS. ALL CELLS WILL BE CULTURED IN THEIR RECOMMENDED MEDIUM WITH NORMAL SERUM SUPPLEMENTS AS A CONTROL, IN A MEDIUM WITH LOW SERUM, AND IN A NUMBER OF CHEMICALLY DEFINED MEDIA. COMPARISONS OF ATTACHMENT AND GROWTH ON PLASMA-TREATED SURFACES AND ON COLLAGEN WILL BE MADE FOR ALL CULTURE CONDTIONS.

* information listed above is at the time of submission.

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