You are here

Organotypic Skin Cultures with Improved Barriers

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 21953
Amount: $749,665.00
Phase: Phase II
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Solicitation Year: N/A
Award Year: 1997
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
200 Homer Avenue
Ashland, MA 01721
United States
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Mitchell Klausner
 (508) 881-6771
Business Contact
Phone: () -
Research Institution

Many highly differentiated in vitro epidermal and dermal/epidermal skin models are being developed which would be extremely useful for 1) transdermal drug delivery systems, 2) skin transplantation, and 3) dermal toxicity testing. However, none of the current models accurately reproduce the barrier properties of human skin. In addition to invalidating barrier studies, this shortcoming is significant since it indicates that the basic keratinocyte cellular metabolism and physiology in vitro is abnormal. Utilizing a serum free keratinocyte culture system, Phase I research will systematically investigate the effect of various media additives chosen specifically to optimize the formation of barrier lipids and epidermal ultrastructure known to be important in the skin barrier function. Phase I experimenta- tion, which will include structural evaluation at the light and electron microscopy levels, lipid analysis, and transepidermal permeability measurements, will demonstrate the utility of the system. These studies will lay the groundwork for Phase II research which will be an in-depth analysis of the nutritional requirements needed to generate skin in vitro with the barrier and function of native skin.

* Information listed above is at the time of submission. *

US Flag An Official Website of the United States Government